CCL3/MIP-1α/LD78α, Human, Recombinant
英文名稱：CCL3/MIP-1α/LD78α, Human, Recombinant
貨號： PCK295
價格： ￥680
規(guī)格：
10μg
產(chǎn)品信息
| 產(chǎn)品形態(tài) | 粉末 |
| 產(chǎn)品規(guī)格 | 10μg |
| 別名 | MIP-1a: Macrophage Inflammatory Protein-1α;LD78α |
| 物種 | 人 |
| 表達宿主 | E.coli |
| 序列信息 | ADTPTACCFSYTSRQIPQNFIADYFETSSQCSKPGVIFLTKRSRQVCADPSEEWVQKYVSDLELSA. |
| 檢索號 | P10147 |
| 分子量 | 8.4 kDa |
| 表觀分子量 | 8-11 kDa |
| 標(biāo)簽 | tag-free |
| 生物活性 | Measure by its ability to chemoattract BaF3 cells transfected with human CCR5. The ED50 for this effect is < 10 ng/mL. |
| 純度 | > 98% as determined by SDS-PAGE. Ni-NTA chromatography. |
| 內(nèi)毒素 | < 0.1 |
| 儲存條件 | Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. |
| 運輸條件 | Ambient temperature or ice pack. |
| 有效期 | 12個月 |
| 制劑 | The protein was lyophilized from a 0.2 μm filtered solution containing 50 mM Tris and 150 mM NaCl, pH 8.5. |
| 復(fù)融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
| 背景介紹 | CCL3 is belonging to the CC chemokine family. CCL3 participates in activating and recruiting cells, such as lymphocytes, monocytes, and granulocytes during acute inflammation. In addition, CCL3 can enhance IFN-γ secretion from activated T cells and thus induces Th1 response, thereby to regulating leukocyte migration. It is reported that CCL3 is involved in susceptibility of HIV infection and disease progression of AIDS. |
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Q1:官網(wǎng)的因子都是不含動物成分的么？
我們公司的因子都是重組的，是不含動物源成分的。
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Q2:細胞因子用無菌水配成了100ug/mL母液，用于實驗時，需要的終濃度為10ng/mL，可以用培養(yǎng)基直接稀釋？還是需要通過PBS（含5%海藻糖）進一步稀釋才行呢？
如果是直接做實驗，可以直接添加培養(yǎng)基進行稀釋配置。 如果是需要長時間保存的話，需要使用含載體蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液進一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細胞因子加無菌水溶解后需要再次離心嗎？
加了無菌水后，如果沒有掛壁現(xiàn)象是不用離心的，如果您放置了一段時間，再次使用時是可以使用離心機離心的
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Q4:細胞因子離心前需要放置室溫嗎？
不用提前放置室溫.
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