Eotaxin-3/CCL26 (24-94), Human, Recombinant
英文名稱：Eotaxin-3/CCL26 (24-94), Human, Recombinant
貨號(hào)： PCK112
價(jià)格： ￥880 ￥2780 ￥9680 ￥13880
產(chǎn)品信息
| 產(chǎn)品形態(tài) | 液體 |
| 產(chǎn)品規(guī)格 | 10μg / 50μg / 500μg / 1mg |
| 別名 | C-C Motif Chemokine 26;CC Chemokine IMAC;Eotaxin-3;Macrophage Inflammatory Protein 4-Alpha;MIP-4-Alpha;Small-Inducible Cytokine A26;Thymic Stroma Chemokine-1;TSC-1;CCL26;SC |
| 物種 | 人 |
| 表達(dá)宿主 | E.coli |
| 序列信息 | Thr24-Leu94 |
| 檢索號(hào) | Q9Y258 |
| 分子量 | 8.53 kDa |
| 表觀分子量 | 13 kDa |
| 純度 | > 95% as determined by reducing SDS-PAGE. |
| 內(nèi)毒素 | < 0.1 |
| 儲(chǔ)存條件 | Store at ≤-70℃, stable for 6 months after receipt. Store at ≤-70℃, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 運(yùn)輸條件 | The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below. |
| 有效期 | 6個(gè)月 |
| 制劑 | Supplied as a 0.2 μm filtered solution of 20 mM Tris, 1 mM EDTA, 20% Glycerol, pH 9.0. |
| 背景介紹 | Chemokine Ligand 26 Protein (CCL26) is a novel small Cytokine belonging to the CC Chemokine familywhich is involved in immunoregulatory and inflammatory processes. CCL26 is constitutively expressed inthymus, but only transiently expressed in phytohemagglutinin-stimulated peripheral blood mononuclearcells. It specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with theChemokine Receptor CCR4. CCL26, along with Eotaxin-1 and Eotaxin-2, selectively activates the CCChemokine Receptor 3 (CCR3). The Eotaxin-3-CCR3 interaction may play an important role in allergicdiseases such as atopic dermatitis and bronchial asthma. The full-length cDNA for CCL26 encodes a Proteinof 94 amino acids with a putative signal peptide of either 23 or 26 amino acid residues. Both the 71 and 68amino acid residue variants of recombinant CCL26 demonstrate equal potency in inducing chemotaxis of ahuman CCR3-transfected cell line. Unlike most other CC Chemokines, CCL26 maps to human chromosome7q11.2, within 40 kilobases of the Eotaxin-2 loci. CCL26 and Eotaxin-2 are unique in that they are the onlyChemokines identified to date that map to chromosome 7. |
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Q1:官網(wǎng)的因子都是不含動(dòng)物成分的么？
我們公司的因子都是重組的，是不含動(dòng)物源成分的。
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Q2:細(xì)胞因子用無菌水配成了100ug/mL母液，用于實(shí)驗(yàn)時(shí)，需要的終濃度為10ng/mL，可以用培養(yǎng)基直接稀釋？還是需要通過PBS（含5%海藻糖）進(jìn)一步稀釋才行呢？
如果是直接做實(shí)驗(yàn)，可以直接添加培養(yǎng)基進(jìn)行稀釋配置。 如果是需要長(zhǎng)時(shí)間保存的話，需要使用含載體蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液進(jìn)一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細(xì)胞因子加無菌水溶解后需要再次離心嗎？
加了無菌水后，如果沒有掛壁現(xiàn)象是不用離心的，如果您放置了一段時(shí)間，再次使用時(shí)是可以使用離心機(jī)離心的
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Q4:細(xì)胞因子離心前需要放置室溫嗎？
不用提前放置室溫.
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Q5:收到細(xì)胞因子產(chǎn)品用什么溶劑復(fù)融？
1、細(xì)胞因子產(chǎn)品大部分為凍干形式發(fā)貨，少部分液體形式發(fā)貨； 2、凍干粉狀態(tài)的因子產(chǎn)品需指定溶劑復(fù)溶，請(qǐng)參考產(chǎn)品說明書【復(fù)融】方法，按照要求使用對(duì)應(yīng)溶劑溶解，大部分因子為無菌水復(fù)融，少部分需要無菌水以外的特殊溶劑溶解的產(chǎn)品則隨貨會(huì)贈(zèng)送相應(yīng)的復(fù)融試劑；凍干粉溶解后如您需要進(jìn)一步稀釋分裝，需使用含載體蛋白(如0.1% BSA，10%FBS，5%HSA 或 5% 海藻糖)的溶液進(jìn)一步稀釋后分裝冷凍保存。 3、液體形式因子產(chǎn)品不需要復(fù)溶，具體濃度詳見產(chǎn)品標(biāo)簽，按照實(shí)驗(yàn)需要用含載體蛋白(如0.1% BSA，10%FBS，5%HSA 或 5% 海藻糖)的溶液進(jìn)一步稀釋后分裝冷凍保存即可；
識(shí)別碼示意圖
