IL-16/LCF, Human, Recombinant
英文名稱:IL-16/LCF, Human, Recombinant
貨號: PCK175
價格: ¥1180 ¥3480 ¥14680 ¥19680
規(guī)格:
10μg
50μg
500μg
1mg
產(chǎn)品信息
產(chǎn)品形態(tài) | 粉末 |
產(chǎn)品規(guī)格 | 10μg / 50μg / 500μg / 1mg |
別名 | LCF (Lymphocyte Chemoattractant Factor) |
物種 | 人 |
表達宿主 | E.coli |
序列信息 | MPDLNSSTDSAASASAASDVSVESTEATVCTVTLEKMSAGLGFSLEGGKGSLHGDKPLTINRIFKGAASEQSETVQPGDEILQLGGTAMQGLTRFEAWNIIKALPDGPVTIVIRRKSLQSKETTAAGDS with polyhistidine tag at the C-terminus. |
檢索號 | Q14005 |
分子量 | 14.12 kDa |
表觀分子量 | 18 kDa |
標簽 | His-tag at the C-terminus |
純度 | > 95% as determined by SDS-PAGE. Ni-NTA chromatography. |
內(nèi)毒素 | < 0.1 |
儲存條件 | Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. |
運輸條件 | Ambient temperature or ice pack. |
有效期 | 12個月 |
制劑 | The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 8.0. |
復融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介紹 | Interleukin 16 (IL-16) is a cytokine that is released by a variety of cells (including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. |
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Q1:官網(wǎng)的因子都是不含動物成分的么?
我們公司的因子都是重組的,是不含動物源成分的。
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Q2:細胞因子用無菌水配成了100ug/mL母液,用于實驗時,需要的終濃度為10ng/mL,可以用培養(yǎng)基直接稀釋?還是需要通過PBS(含5%海藻糖)進一步稀釋才行呢?
如果是直接做實驗,可以直接添加培養(yǎng)基進行稀釋配置。 如果是需要長時間保存的話,需要使用含載體蛋白(如0.1%BSA,10% FBS,5%HSA或5%海藻糖)的溶液進一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細胞因子加無菌水溶解后需要再次離心嗎?
加了無菌水后,如果沒有掛壁現(xiàn)象是不用離心的,如果您放置了一段時間,再次使用時是可以使用離心機離心的
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Q4:細胞因子離心前需要放置室溫嗎?
不用提前放置室溫.
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Q5:因子到貨了,里面有兩管溶液,一個是水,一個是海藻糖溶液,用哪一個稀釋呢?
1、將裝有細胞因子的管子放入小離心機內(nèi)短暫離心20~30s(離心機最大轉(zhuǎn)速即可),使附著在管壁的微量蛋白聚集在管底; 2、先用無菌水溶解因子,很少量水即可溶解因子,注意計算溶解后濃度不低于100 μg/mL; 3、用贈送的含海藻糖的PBS溶液進一步稀釋到您實驗合適的濃度即可,然后分裝后放入-20℃以下保藏; 4、因子復融重懸過程應(yīng)輕柔,不可以震蕩渦旋。
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