IL-27 (C-6His), Mouse, Recombinant
英文名稱：IL-27 (C-6His), Mouse, Recombinant
貨號(hào)： PCK258
價(jià)格： ￥1480 ￥4280 ￥19680 ￥27880
規(guī)格：
10μg
50μg
500μg
1mg
產(chǎn)品信息
| 產(chǎn)品形態(tài) | 粉末 |
| 產(chǎn)品規(guī)格 | 10μg / 50μg / 500μg / 1mg |
| 別名 | Interleukin-27 subunit alpha;IL-27 subunit alpha;IL-27-A;IL27-A;p28;Il27;Il27a;Interleukin-27 subunit beta;Ebi3;IL-27 subunit beta;IL-27B;Epstein-Barr virus-induced gene 3 Protein homolog;Ebi3;Il27b |
| 物種 | 小鼠 |
| 表達(dá)宿主 | Human Cells |
| 序列信息 | Tyr19-Pro228&Phe29-Ser234 |
| 檢索號(hào) | O35228&Q8K3I6 |
| 分子量 | 49 kDa |
| 表觀分子量 | 60-65 kDa |
| 標(biāo)簽 | C-6His |
| 純度 | > 90% as determined by reducing SDS-PAGE. |
| 內(nèi)毒素 | < 0.1 |
| 儲(chǔ)存條件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 運(yùn)輸條件 | Ambient temperature or ice pack. |
| 有效期 | 12個(gè)月 |
| 制劑 | Lyophilized from a 0.2 μm filtered solution of PBS, 2 mM EDTA, 5% Trehalose, pH 7.4. |
| 復(fù)融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 背景介紹 | IL-27 is a heterodimeric Cytokine which belongs to the IL-6/IL-12 family of long type I Cytokines. It is expressed on monocytes, endothelial cells and dendritic cells. IL-27 is an early product of activated antigen-presenting cells and drives rapid clonal expansion of naive CD4 (+) T cells and plays a role in the early regulation of Th1 cells initiation which drives efficient adaptive immune response. IL-27 potentiates the early phase of TH1 response and suppresses TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21-and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. IL-27 has an antiproliferative activity on melanomas through WSX-1/STAT1 signaling. Thus, IL-27 Protein may be an attractive candidate as an antitumor agent applicable to cancer immunotherapy. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL27 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. IL-27 has also an effect on Cytokine production. It suppresses proinflammatory Cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of Cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of Cytokine production, IL-27 also antagonizes the effects of some Cytokines such as IL6 through direct effects on T-cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic Chemokines such as IP-10/CXCL10 and MIG/CXCL9. |
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Q1:官網(wǎng)的因子都是不含動(dòng)物成分的么？
我們公司的因子都是重組的，是不含動(dòng)物源成分的。
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Q2:細(xì)胞因子用無(wú)菌水配成了100ug/mL母液，用于實(shí)驗(yàn)時(shí)，需要的終濃度為10ng/mL，可以用培養(yǎng)基直接稀釋？還是需要通過PBS（含5%海藻糖）進(jìn)一步稀釋才行呢？
如果是直接做實(shí)驗(yàn)，可以直接添加培養(yǎng)基進(jìn)行稀釋配置。 如果是需要長(zhǎng)時(shí)間保存的話，需要使用含載體蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液進(jìn)一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細(xì)胞因子加無(wú)菌水溶解后需要再次離心嗎？
加了無(wú)菌水后，如果沒有掛壁現(xiàn)象是不用離心的，如果您放置了一段時(shí)間，再次使用時(shí)是可以使用離心機(jī)離心的
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Q4:細(xì)胞因子離心前需要放置室溫嗎？
不用提前放置室溫.
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