IL-2RB/CD122 (C-6His), Human, Recombinant
英文名稱:IL-2RB/CD122 (C-6His), Human, Recombinant
貨號: PCK151
價格: ¥1180 ¥3480 ¥11880 ¥18880
規(guī)格:
10μg
50μg
500μg
1mg
產(chǎn)品信息
產(chǎn)品形態(tài) | 粉末 |
產(chǎn)品規(guī)格 | 10μg / 50μg / 500μg / 1mg |
別名 | Interleukin-2 Receptor subunit beta;IL2RB;IL-2 Receptor subunit beta;IL-2R subunit beta;High affinity IL-2 Receptor subunit beta;CD122 |
物種 | 人 |
表達宿主 | Human Cells |
序列信息 | Ala27-Asp239 |
檢索號 | P14784 |
分子量 | 25.6 kDa |
表觀分子量 | 35-40 kDa |
標簽 | C-6His |
生物活性 | Loaded Human IL-2RB-His on HIS1K Biosensor, can bind Human IL-2 with an affinity constant of 0.27 μM as determined in BLI assay. |
純度 | > 95% as determined by reducing SDS-PAGE. |
內(nèi)毒素 | < 0.1 |
儲存條件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
運輸條件 | Ambient temperature or ice pack. |
有效期 | 12個月 |
制劑 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
復融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
背景介紹 | Human IL-2RB, also known asInterleukin-2 Receptor subunit beta,is the Receptor for Interleukin-2. IL2 Receptor complex is involved in Receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2 Receptor complex has three forms with respect to ability to bind IL2. IL-2RB is belonged to a type I membrane Protein,and has a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. IL-2RB is the subunit critical for Receptor-mediated signaling via physically or functionally coupling to other signaling molecules, such as the Jak-STAT and Src-family Protein tyrosine kinase although it lacks apparent catalytic Motifs. |
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Q1:官網(wǎng)的因子都是不含動物成分的么?
我們公司的因子都是重組的,是不含動物源成分的。
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Q2:細胞因子用無菌水配成了100ug/mL母液,用于實驗時,需要的終濃度為10ng/mL,可以用培養(yǎng)基直接稀釋?還是需要通過PBS(含5%海藻糖)進一步稀釋才行呢?
如果是直接做實驗,可以直接添加培養(yǎng)基進行稀釋配置。 如果是需要長時間保存的話,需要使用含載體蛋白(如0.1%BSA,10% FBS,5%HSA或5%海藻糖)的溶液進一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細胞因子加無菌水溶解后需要再次離心嗎?
加了無菌水后,如果沒有掛壁現(xiàn)象是不用離心的,如果您放置了一段時間,再次使用時是可以使用離心機離心的
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Q4:細胞因子離心前需要放置室溫嗎?
不用提前放置室溫.

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