RANK/TNFRSF11A/CD265 (C-6His), Mouse, Recombinant
英文名稱：RANK/TNFRSF11A/CD265 (C-6His), Mouse, Recombinant
貨號： PCK059
價格： ￥780 ￥1880 ￥11880 ￥17280
產品信息
| 產品形態(tài) | 粉末 |
| 產品規(guī)格 | 10μg / 50μg / 500μg / 1mg |
| 別名 | Receptor activator of NF-KB;tumor necrosis factor Receptor superfamily member 11A;TRANCE Receptor;Osteoclast differentiation factor Receptor;NFKB activator;TRANCER;CD265;TNFRSF11A;TRANCE R;CD265 antigen;ODFR |
| 物種 | 小鼠 |
| 表達宿主 | Human Cells |
| 序列信息 | Val31-Ser214 |
| 檢索號 | O35305 |
| 分子量 | 21.3 kDa |
| 表觀分子量 | 26-30 kDa |
| 標簽 | C-6His |
| 純度 | > 95% as determined by reducing SDS-PAGE. |
| 內毒素 | < 0.1 |
| 儲存條件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 運輸條件 | Ambient temperature or ice pack. |
| 有效期 | 12個月 |
| 制劑 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 復融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in sterile water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 背景介紹 | Receptor activator of NF-κB (RANK, TNFRSF11A) belongs to one member of tumor necrosis factor Receptor family.It is a Receptor for TNFSF11/RANKL/TRANCE/OPGL. This gene encodes a type 1 membrane Protein with a 30 amino acids (aa) signal peptide, 184 aa extracellular region, a 20 aa transmembrane domain and a 391 aa cytoplasmic region. Human and murine RANK share 81% aa identity in their extracellular domains. RANK is ubiquitous highly expressed in trabecular bone, thymus, small intestine, lung, brain and kidney, but weakly expressed in spleen and bone marrow. After binding its Ligand RANKL, RANK can activate signaling pathways such as NF-κB, JNK, ERK, p38, and Akt/PKB, through TRAF Protein phosphorylation. RANK/TNFRSF11A signaling is largely considered to be growth promoting and apoptosis reducing such as the effects observed in osteoclasts. RANK/TNFRSF11A was also found to be involved in the regulation of interactions between T-cells and dendritic cells. |
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Q1:官網的因子都是不含動物成分的么？
我們公司的因子都是重組的，是不含動物源成分的。
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Q2:細胞因子用無菌水配成了100ug/mL母液，用于實驗時，需要的終濃度為10ng/mL，可以用培養(yǎng)基直接稀釋？還是需要通過PBS（含5%海藻糖）進一步稀釋才行呢？
如果是直接做實驗，可以直接添加培養(yǎng)基進行稀釋配置。 如果是需要長時間保存的話，需要使用含載體蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液進一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細胞因子加無菌水溶解后需要再次離心嗎？
加了無菌水后，如果沒有掛壁現象是不用離心的，如果您放置了一段時間，再次使用時是可以使用離心機離心的
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Q4:細胞因子離心前需要放置室溫嗎？
不用提前放置室溫.
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Q5:收到細胞因子產品用什么溶劑復融？
1、細胞因子產品大部分為凍干形式發(fā)貨，少部分液體形式發(fā)貨； 2、凍干粉狀態(tài)的因子產品需指定溶劑復溶，請參考產品說明書【復融】方法，按照要求使用對應溶劑溶解，大部分因子為無菌水復融，少部分需要無菌水以外的特殊溶劑溶解的產品則隨貨會贈送相應的復融試劑；凍干粉溶解后如您需要進一步稀釋分裝，需使用含載體蛋白(如0.1% BSA，10%FBS，5%HSA 或 5% 海藻糖)的溶液進一步稀釋后分裝冷凍保存。 3、液體形式因子產品不需要復溶，具體濃度詳見產品標簽，按照實驗需要用含載體蛋白(如0.1% BSA，10%FBS，5%HSA 或 5% 海藻糖)的溶液進一步稀釋后分裝冷凍保存即可；
識別碼示意圖
