TPO/MGDF/THPO, Human, Recombinant
英文名稱：TPO/MGDF/THPO, Human, Recombinant
貨號： PCK366
價格： ￥680 ￥1680
規(guī)格：
10μg
50μg
產(chǎn)品信息
| 產(chǎn)品形態(tài) | 粉末 |
| 產(chǎn)品規(guī)格 | 10μg / 50μg |
| 別名 | Megakaryocyte colony-stimulating factor;c-MPL Ligand;MGDF;THPO |
| 物種 | 人 |
| 表達(dá)宿主 | E.coli |
| 序列信息 | SPAPPACDLRVLSKLLRDSHVLHSRLSQCPEVHPLPTPVLLPAVDFSLGEWKTQMEETKAQDILGAVTLLLEGVMAARGQLGPTCLSSLLGQLSGQVRLLLGALQSLLGTQLPPQGRTTAHKDPNAIFLSFQHLLRGKVRFLMLVGGSTLCVRRAPPTTAVPSRTSLVLTLNEL with polyhistidine tag at the N-terminus. |
| 檢索號 | P40225.1 |
| 分子量 | 19.6 kDa |
| 表觀分子量 | 20 kDa |
| 標(biāo)簽 | His-tag at the N-terminus |
| 生物活性 | Measure by its ability to induce proliferation in MO7e cells. The ED50 for this effect is < 2 ng/mL. The specific activity of recombinant human TPO is > 5 × 10^5 IU/mg. |
| 純度 | > 95% as determined by SDS-PAGE. Ni-NTA chromatography. |
| 內(nèi)毒素 | < 0.1 |
| 儲存條件 | Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. |
| 運(yùn)輸條件 | Ambient temperature or ice pack. |
| 有效期 | 12個月 |
| 制劑 | The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 7.4. |
| 復(fù)融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
| 背景介紹 | Thrombopoietin is a glycoprotein hormone produced mainly by the liver and the kidney that regulates the production of platelets by the bone marrow. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. It stimulates the production and differentiation of megakaryocytes, the bone marrow cells that fragment into large numbers of platelets. |
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Q1:官網(wǎng)的因子都是不含動物成分的么？
我們公司的因子都是重組的，是不含動物源成分的。
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Q2:細(xì)胞因子用無菌水配成了100ug/mL母液，用于實(shí)驗(yàn)時，需要的終濃度為10ng/mL，可以用培養(yǎng)基直接稀釋？還是需要通過PBS（含5%海藻糖）進(jìn)一步稀釋才行呢？
如果是直接做實(shí)驗(yàn)，可以直接添加培養(yǎng)基進(jìn)行稀釋配置。 如果是需要長時間保存的話，需要使用含載體蛋白(如0.1%BSA，10% FBS，5%HSA或5%海藻糖)的溶液進(jìn)一步釋后分裝保存于-5~-20℃或者-80℃冰箱
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Q3:細(xì)胞因子加無菌水溶解后需要再次離心嗎？
加了無菌水后，如果沒有掛壁現(xiàn)象是不用離心的，如果您放置了一段時間，再次使用時是可以使用離心機(jī)離心的
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Q4:細(xì)胞因子離心前需要放置室溫嗎？
不用提前放置室溫.
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