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產(chǎn)品概述
產(chǎn)品信息
| 名稱 | SK-N-DZ (人神經(jīng)母細(xì)胞瘤細(xì)胞) (STR鑒定正確) |
| 別稱 | SKNDZ |
| 種屬 | 人 |
| 生長特性 | 貼壁細(xì)胞 |
| 細(xì)胞形態(tài) | 成神經(jīng)細(xì)胞樣 |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認(rèn)） |
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
|
| 推薦傳代比例 | 1:3-1:4 |
| 推薦換液頻率 | 2-3次/周 |
| 注意事項 | 該細(xì)胞貼壁松散，操作時請盡量輕柔；換液時需預(yù)熱培養(yǎng)基；收貨如有大塊脫落或全部脫落的細(xì)胞團(tuán)，為正常現(xiàn)象，請按照收貨注意事項處理；細(xì)胞離心復(fù)蘇之后有少量漂浮，第二天換液即可，每三天換液一次，等細(xì)胞密度達(dá)到80%左右，按照1:2進(jìn)行傳代，傳代之后有少量漂浮，換液即可，消化時間在顯微鏡下觀察，以細(xì)胞變圓，脫落為主，大約控制消化時間在4-6mim左右。 |
參考資料(來源文獻(xiàn))
| 背景描述 | SK-N-DZ are neuroblasts that were isolated from the brain of a 2-year-old, White female patient with neuroblastoma.SK-N-DZ are neuroblasts that were isolated from the brain of a 2-year-old, White female patient with neuroblastoma. Expression of the N-myc gene product was reduced in differentiated SK-N-DZ cells as compared with undifferentiated cells. Expression of the c-src gene product, pp60c-src was enhanced in differentiated SK-N-DZ cells as was tyrosine phosphorylation of cellular proteins. The cells exhibit moderate MDR1 expression. Retinoic acid induces differentiation in this line. |
| 年齡（性別） | 女性；2歲 |
| 組織來源 | 大腦 |
| 細(xì)胞類型 | 腫瘤細(xì)胞 |
| 腫瘤類型 | 神經(jīng)母細(xì)胞瘤細(xì)胞 |
| 生物安全等級 | BSL-1 |
| 致瘤性 | Yes; Yes, forms tumors in nude mice |
| 保藏機構(gòu) | ATCC; CRL-2149 |
STR鑒定
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STR位點信息
Amelogenin X CSF1PO 12 D2S1338 21,22,23 D3S1358 17 D5S818 12 D7S820 12,13 D8S1179 12,13 D13S317 8,11 D16S539 9,11 D18S51 15,17 D19S433 14.2,16 D21S11 29,31 FGA 18,22 PentaD 9,14 PentaE 7,10 TH01 6,9 TPOX 8 vWA 16,18 D6S1043 14,19 D12S391 18,21 D2S441 10,11 -
STR鑒定圖
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