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產(chǎn)品概述
產(chǎn)品信息
| 名稱 | NCI-H526 (人小細胞肺癌細胞) (STR鑒定正確) |
| 別稱 | H526; H-526; NCIH526 |
| 種屬 | 人 |
| 生長特性 | 懸浮細胞 |
| 細胞形態(tài) | 圓形聚團 |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認） |
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
|
| 推薦傳代比例 | 1:3-1:6 |
| 推薦換液頻率 | 2-3次/周 |
參考資料(來源文獻)
| 背景描述 | NCI-H526 [H526] is an epithelial cell line that was isolated from the lungs of a 55-year-old, White male with carcinoma.NCI-H526 expresses elevated levels of 2 biochemical markers of SCLC: neuron specific enolase and the brain isoenzyme of creatine kinase. They do not express L-dopa carboxylase or bombesin-like immunoreactivity. These cells express the c-kit gene as well as the N-myc gene, but not c-myc or L-myc. N-myc is amplified and p75 c-myb expression was observed. NCI-H526 also expresses the proto-oncogenes N-ras, Ki-ras, Ha-ras, and c-raf1. Only trace amounts of the retinoblastoma susceptibility gene, RB mRNA were detected. RB protein was not detected. The cells express easily detectable levels of p53 mRNA compared to levels found in normal lung. Abnormally sized mRNA was present. |
| 年齡（性別） | 男性；55歲 |
| 組織來源 | 肺 |
| 細胞類型 | 腫瘤細胞 |
| 腫瘤類型 | 小細胞肺癌 |
| 生物安全等級 | BSL-1 |
| 倍增時間 | 36-48 hours |
| 致瘤性 | Yes; Yes, the cells produce tumors in athymic nude mice |
| 保藏機構(gòu) | ATCC; CRL-5811 |
STR鑒定
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STR位點信息
Amelogenin X CSF1PO 11 D2S1338 23,24 D3S1358 17 D5S818 11 D7S820 9,12 D8S1179 14 D13S317 13 D16S539 9 D18S51 12,15 D19S433 13,15 D21S11 27,32.2 FGA 22 PentaD 13,14 PentaE 5,13 TH01 6,8 TPOX 8,11 vWA 16,17 D6S1043 11 D12S391 15,18 D2S441 11,14 -
STR鑒定圖
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