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產品概述
產品信息
名稱 | HS-27A (人骨髓細胞) (STR鑒定正確) |
別稱 | HS27A; HS27a |
種屬 | 人類 |
生長特性 | 貼壁細胞 |
細胞形態(tài) | 成纖維細胞樣 |
凍存條件 | 凍存液:55% 基礎培養(yǎng)基+40% FBS+5% DMSO 溫度:液氮 |
培養(yǎng)方案A(默認) |
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 1:2-1:3 |
推薦換液頻率 | 2-3次/周 |
參考資料(來源文獻)
背景描述 | HS-27A is a fibroblast cell line that was isolated in (year from History) from the stroma of a 30-year-old White male donor. HS-27A is a subclone of HS-27.HS-27A forms large flattened polygonal shaped cells that exemplify "blanket" cells and maintain numerous intercellular contacts with neighboring cells. Unlike HS-5, HS-27A secretes low levels of growth factors and does not support proliferation of isolated progenitor cells in cocultures. HS-27A expresses relatively high levels of vascular cell adhesion molecule 1 (VCAM-1). |
年齡(性別) | 男性;30歲 |
組織來源 | 骨頭;骨髓;基質 |
細胞類型 | 轉化細胞系 |
生物安全等級 | BSL-2 |
倍增時間 | ~36h-48 hours |
抗原表達情況 | Vascular cell adhesion molecule 1 (CD106, VCAM-1) |
應用領域 | 3D cell culture; Immunology |
保藏機構 | ATCC; CRL-2496 |
STR鑒定
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STR位點信息
Amelogenin X, Y CSF1PO 10,11 D2S1338 22 D3S1358 14,17 D5S818 12 D7S820 12 D8S1179 13,16 D13S317 11,13 D16S539 11,12 D18S51 12,15 D19S433 15 D21S11 29,30 FGA 21,24 PentaD 10,13 PentaE 5,6 TH01 7,9 TPOX 8,11 vWA 18,19 D6S1043 15,18 D12S391 19,21 D2S441 11 -
STR鑒定圖
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