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產(chǎn)品概述
產(chǎn)品信息
| 名稱 | DoHH2 (人B細(xì)胞淋巴瘤細(xì)胞) (STR鑒定正確) |
| 別稱 | DOHH2; DoHH-2; DOHH-2 |
| 種屬 | 人源 |
| 生長特性 | 懸浮細(xì)胞 |
| 細(xì)胞形態(tài) | 淋巴細(xì)胞樣 |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40% FBS+5% DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認(rèn)） |
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
|
| 推薦傳代比例 | 1:2-1:4 |
| 推薦換液頻率 | 2-3次/周 |
參考資料(來源文獻(xiàn))
| 背景描述 | established from the pleural effusion of a 60-year-old man with refractory immunoblastic B cell lymphoma progressed from follicular centroblastic/centrocytic lymphoma in 1990; cells were described in the literature to carry the t(14;18) leading to IGH-BCL2 fusion gene and to express BCL2 mRNA; the original culture was a mixture of EBV - and EBV + cells, of which the EBV - cells were isolated and clonally expanded. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| 年齡（性別） | 男性；60歲 |
| 組織來源 | 胸腔積液 |
| 細(xì)胞類型 | 腫瘤細(xì)胞 |
| 腫瘤類型 | 淋巴瘤細(xì)胞 |
| 生物安全等級 | BSL-1 |
| 倍增時(shí)間 | ~40 hours |
| 保藏機(jī)構(gòu) | DSMZ; ACC-47 |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X CSF1PO 11,12 D2S1338 22,24 D3S1358 15,17 D5S818 11,12 D7S820 11 D8S1179 13,16 D13S317 12,13 D16S539 11,14 D18S51 11,14 D19S433 14.2,15.2 D21S11 30 FGA 21,27 PentaD 9,12 PentaE 12,16 TH01 9 TPOX 8,11 vWA 14,19 D6S1043 11,12 D12S391 19,20 D2S441 11,14 -
STR鑒定圖
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