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VEGF165/VEGF-A, Human, Recombinant
貨號(hào)：PCK267
價(jià)格：￥980￥3180￥5180￥13880
規(guī)格：
10μg
- 10μg
- 50μg
- 100μg
- 500μg
產(chǎn)品概述
產(chǎn)品信息
| 別名 | VPF, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen |
| 物種 | Human |
| 表達(dá)宿主 | E.coli |
| 序列信息 | MAPMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR with polyhistidine tag at the C-terminus. |
| 檢索號(hào) | P15692.2 |
| 分子量 | 20.11 kDa |
| 表觀分子量 | 21 kDa |
| 標(biāo)簽 | His-tag?at?the?C-terminus |
| 生物活性 | Measure by its ability to induce HUVEC cells proliferation.The ED50 for this effect is <5 ng/mL.The specific activity of recombinant human VEGF165 is approximately >1.4 x 10^6 IU/mg. |
產(chǎn)品特性
| 純度 | >98% as determined by SDS-PAGE. Ni-NTA chromatography |
| 內(nèi)毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1%BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
| 運(yùn)輸 | Ambient temperature or ice pack. |
| 制劑 | The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 8.0. |
| 復(fù)融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介紹
Vascular endothelial growth factor (VEGF), originally known as vascular permeability factor (VPF), is a signal protein produced by cells that stimulates the formation of blood vessels. VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M and TNF-alpha.
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