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產(chǎn)品概述
名稱 | Ba/F3 (小鼠原B細胞) (STR鑒定正確) |
別稱 | BA/F3; BaF3; BAF3; Baf3 |
種屬 | 小鼠 |
生長特性 | 懸浮細胞 |
細胞形態(tài) | 圓形 |
凍存條件 | 凍存液:90%FBS+10%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認) |
培養(yǎng)條件:
氣相:空氣,95%;CO?,5%, 溫度:37℃
|
推薦傳代比例 | 3×10^5-1×10^6個/mL |
推薦換液頻率 | 2-3次/周 |
背景描述 | IL-3 dependent murine pro B cell line; an original publication describing establishment of BA/F3 does not exist; probably a subclone of a first described Ea3 cell line. Performing May-Gruenwald-Giemsa staining provides evidence that the cells are indeed from a lymphatic background, however cells were apparently not derived from BALB/c, but from C3H mouse. |
組織來源 | 骨髓 |
細胞類型 | 因子依賴細胞系 |
生物安全等級 | BSL-1 |
倍增時間 | ~20 hours |
保藏機構(gòu) | DSMZ; ACC-300 |
STR鑒定
-
STR位點信息
18-3 16 6-7 12 5-5 15,17 X-1 26,27 1-2 16 7-1 26 8-1 16 1-1 10 19-2 11,12 15-3 24.3 12-1 16 6-4 18,19 4-2 19.3 3-2 14 2-1 9 13-1 17.1
11-2 16 17-2 16 -
STR鑒定圖
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