產(chǎn)品中心Cell Resources

特色產(chǎn)品

細(xì)胞資源庫(kù)

細(xì)胞培養(yǎng)基

輔助試劑

細(xì)胞功能研究試劑盒

聯(lián)系我們CONTACT US

400-999-210024小時(shí)服務(wù)熱線
sales@procell.com.cn銷(xiāo)售郵箱

IL-1H4, Human, Recombinant
一鍵復(fù)制產(chǎn)品信息

貨號(hào)：PCK196

價(jià)格：￥1780￥4980￥17480￥25680

規(guī)格：

10μg

10μg
50μg
500μg
1mg

數(shù)量： - +

說(shuō)明書(shū)

在線咨詢(xún)

產(chǎn)品概述

產(chǎn)品信息

別名	IL-1F7, IL-1ζ (zeta), IL-1H4, FIL1, FIL1Z
物種	Human
表達(dá)宿主	E.coli
序列信息	MKNLNPKKFSIHDQDHKVLVLDSGNLIAVPDKNYIRPEIFFALASSLSSASAEKGSPILLGVSKGEFCLYCDKDKGQSHPSLQLKKEKLMKLAAQKESARRPFIFYRAQVGSWNMLESAAHPGWFICTSCNCNEPVGVTDKFENRKHIEFSFQPVCKAEMSPSEVSD with polyhistidine tag at the C-terminus.
檢索號(hào)	Q9NZH6.1
分子量	19.49 kDa
表觀分子量	22 kDa
標(biāo)簽	His-tag?at?the?C-terminus
生物活性	Measure by its ability to induce IL-8 secretion in human PBMCs.The ED50 for this effect is <0.9 μg/mL.

產(chǎn)品特性

純度	>95% as determined by SDS-PAGE. Ni-NTA chromatography.
內(nèi)毒素	<0.1 EU per 1 μg of the protein by the LAL method.
保存	Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1% BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months.
運(yùn)輸	Ambient temperature or ice pack.
制劑	The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 8.0.
復(fù)融	It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

背景介紹

Decreasing synthesis of IL-37 in PBMCs will cause increasing production of pro-inflammatory cytokines such as IL-1 alpha, IL-1 beta, IL-6 and TNF- alpha. The IL-37 work as an innate inflammatory inhibitor is also proved by its high expression in synovial tissue from rheumatoid arthritis patients. Full length IL-37 locates mainly in the cytoplasm, and it can translocate to the nucleus after activation through cleavage by CASP1, then exerts its activity by directly interacting with SMAD3.