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IL-8/MDNCF/NAF/CXCL8, Human, Recombinant
貨號(hào)：PCK204
價(jià)格：￥1280￥3380￥13880￥19980
規(guī)格：
10μg
- 10μg
- 50μg
- 500μg
- 1mg
產(chǎn)品概述
產(chǎn)品信息
| 別名 | Monocyte-Derived Neutrophil Chemotactic Factor (MDNCF), Neutrophil Activating Factor (NAF), CXCL8, C-X-C motif chemokine ligand 8 |
| 物種 | Human |
| 表達(dá)宿主 | E.coli |
| 序列信息 | MSAKELRCQCIKTYSKPFHPKFIKELRVIESGPHCANTEIIVKLSDGRELCLDPKENWVQRVVEKFLKRAENS with polyhistidine tag at the C-terminus. |
| 檢索號(hào) | P10145.1 |
| 分子量 | 9.32 kDa |
| 表觀分子量 | 11 kDa |
| 標(biāo)簽 | His-tag?at?the?C-terminus |
| 生物活性 | Measure by its ability to chemoattract PBMC. The ED50 for this effect is <2 ng/mL. |
產(chǎn)品特性
| 純度 | >98% as determined by SDS-PAGE. Ni-NTA chromatography |
| 內(nèi)毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1% BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
| 運(yùn)輸 | Ambient temperature or ice pack. |
| 制劑 | The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 8.0. |
| 復(fù)融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介紹
Interleukin 8 (IL8 or chemokine (C-X-C motif) ligand 8, CXCL8) is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells, and endothelial cells. Endothelial cells store IL-8 in their storage vesicles, the Weibel-Palade bodies. In humans, the interleukin-8 protein is encoded by the CXCL8 gene. IL-8 is initially produced as a precursor peptide of 99 amino acids which then undergoes cleavage to create several active IL-8 isoforms. In culture, a 72 amino acid peptide is the major form secreted by macrophages.
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