GM-CSF/CSF2/CSF, Human, Recombinant
英文名稱：GM-CSF/CSF2/CSF, Human, Recombinant
貨號(hào)： PCK035
價(jià)格： ￥9680 ￥2780 ￥880 ￥13880
產(chǎn)品信息
| 產(chǎn)品形態(tài) | 粉末 |
| 產(chǎn)品規(guī)格 | 500μg / 50μg / 10μg / 1mg |
| 別名 | colony stimulating factor 2;CSF2;CSF |
| 物種 | 人 |
| 表達(dá)宿主 | E.coli |
| 序列信息 | APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE with polyhistidine tag at the N-terminus. |
| 檢索號(hào) | P04141.1 |
| 分子量 | 15.4 kDa |
| 表觀分子量 | 15 kDa |
| 標(biāo)簽 | His-tag at the N-terminus |
| 生物活性 | His-tag at the N-terminus |
| 純度 | > 98% as determined by SDS-PAGE. Ni-NTA chromatography. |
| 內(nèi)毒素 | < 0.1 EU per 1 μg of the protein by the LAL method. |
| 儲(chǔ)存條件 | Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. |
| 運(yùn)輸條件 | Ambient temperature or ice pack. |
| 有效期 | 12個(gè)月 |
| 制劑 | The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 8.0. |
| 復(fù)融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
| 背景介紹 | Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. GM-CSF also plays a role in embryonic development by functioning as an embryokine produced by reproductive tract. |
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Q1:因子到貨了，里面有兩管溶液，一個(gè)是水，一個(gè)是海藻糖溶液，用哪一個(gè)稀釋呢？
1、將裝有細(xì)胞因子的管子放入小離心機(jī)內(nèi)短暫離心20~30s（離心機(jī)最大轉(zhuǎn)速即可），使附著在管壁的微量蛋白聚集在管底； 2、先用無菌水溶解因子，很少量水即可溶解因子，注意計(jì)算溶解后濃度不低于100 μg/mL； 3、用贈(zèng)送的含海藻糖的PBS溶液進(jìn)一步稀釋到您實(shí)驗(yàn)合適的濃度即可，然后分裝后放入-20℃以下保藏； 4、因子復(fù)融重懸過程應(yīng)輕柔，不可以震蕩渦旋。
識(shí)別碼示意圖
