IL-2/TCGF/Aldesleukin, Mouse, Recombinant
英文名稱：IL-2/TCGF/Aldesleukin, Mouse, Recombinant
貨號： PCK025
價格： ￥11880 ￥3080 ￥2080 ￥580 ￥17280
產(chǎn)品信息
| 產(chǎn)品形態(tài) | 粉末 |
| 產(chǎn)品規(guī)格 | 500μg / 100μg / 50μg / 10μg / 1mg |
| 別名 | T-cell Growth Factor (TCGF);Aldesleukin |
| 物種 | 小鼠 |
| 表達宿主 | E.coli |
| 序列信息 | MAPTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIISTSPQ with polyhistidine tag at the C-terminus. |
| 檢索號 | P04351.1 |
| 分子量 | 18.2 kDa |
| 表觀分子量 | 18 kDa |
| 標簽 | His-tag at the C-terminus |
| 生物活性 | His-tag at the C-terminus |
| 純度 | > 98% as determined by SDS-PAGE. Ni-NTA chromatography. |
| 內(nèi)毒素 | < 0.1 EU per 1 μg of the protein by the LAL method. |
| 儲存條件 | Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months. |
| 運輸條件 | Ambient temperature or ice pack. |
| 有效期 | 12個月 |
| 制劑 | The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 7.4. |
| 復(fù)融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
| 背景介紹 | Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 -16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes. |
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Q1:因子到貨了，里面有兩管溶液，一個是水，一個是海藻糖溶液，用哪一個稀釋呢？
1、將裝有細胞因子的管子放入小離心機內(nèi)短暫離心20~30s（離心機最大轉(zhuǎn)速即可），使附著在管壁的微量蛋白聚集在管底； 2、先用無菌水溶解因子，很少量水即可溶解因子，注意計算溶解后濃度不低于100 μg/mL； 3、用贈送的含海藻糖的PBS溶液進一步稀釋到您實驗合適的濃度即可，然后分裝后放入-20℃以下保藏； 4、因子復(fù)融重懸過程應(yīng)輕柔，不可以震蕩渦旋。
識別碼示意圖
