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產(chǎn)品概述
產(chǎn)品信息
| 名稱 | U-2932 (人彌漫大B淋巴瘤細(xì)胞) (STR鑒定正確) |
| 別稱 | U2932 |
| 種屬 | 人 |
| 生長特性 | 懸浮細(xì)胞 |
| 細(xì)胞形態(tài) | 淋巴母細(xì)胞樣，圓形，單個(gè)細(xì)胞，少數(shù)聚團(tuán) |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認(rèn)） |
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
|
| 推薦傳代比例 | 1:3-1:4 |
| 推薦換液頻率 | 2-3次/周 |
參考資料(來源文獻(xiàn))
| 背景描述 | established in 1996 from the ascites of a 29-year-old woman with diffuse large B cell lymphoma, who 16 years earlier was diagnosed with advanced stage Hodgkin lymphoma, and relapsed several times after multiple chemo- and radiotharapy regimens to complete remissions; cells were described in the literature to overexpress BCL2, BCL6 and p53; assigned to ABC-like lymphoma subtype (activated B-cell). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq). Also, please note: the cell line U-2932 harbours two distinct subpopulations as visible by surface staining of CD19, CD20, and CD38 (see ref 18133); continuous monitoring of subpopulations is advised to prevent accidental loss during passaging |
| 年齡（性別） | 女性；29歲 |
| 組織來源 | 腹水 |
| 細(xì)胞類型 | 腫瘤細(xì)胞 |
| 腫瘤類型 | 淋巴瘤細(xì)胞 |
| 生物安全等級 | BSL-1 |
| 倍增時(shí)間 | 48 hours (PubMed=12533045); ~50 hours (DSMZ=ACC-633) |
| 保藏機(jī)構(gòu) | DSMZ; ACC-633 |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X CSF1PO 10,12 D2S1338 22,23 D3S1358 15,16 D5S818 12 D7S820 9,13 D8S1179 13,14 D13S317 11 D16S539 12 D18S51 15 D19S433 14 D21S11 28,30 FGA 22,24 PentaD 14 PentaE 13,14 TH01 9.3 TPOX 11,12 vWA 14,17 D6S1043 18 D12S391 18,20 D2S441 12,15 -
STR鑒定圖
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