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Hank's平衡鹽溶液(HBSS),含鈣、鎂,不含酚紅
貨號(hào):PB180323
價(jià)格:¥50¥¥¥
- 500mL
產(chǎn)品概述
平衡鹽溶液(Balanced Salt Solution, BSS)也叫生理溶液(Physiological Solution),是集緩沖液的緩沖力、生理鹽水的等滲性以及培養(yǎng)液的營(yíng)養(yǎng)供應(yīng)特點(diǎn)于一體的,具有維持滲透壓、保持PH穩(wěn)定以及提供簡(jiǎn)單的營(yíng)養(yǎng)的作用,可滿足體外實(shí)驗(yàn)中組織、器官或細(xì)胞的生存和代謝的基本需要。一些平衡鹽溶液中還添加了少量酚紅,用以指示溶液的酸堿度變化。
Hank's平衡鹽溶液(Hank's Balanced Salt Solution, HBSS)是細(xì)胞分離或培養(yǎng)中常用的磷酸鹽緩沖液之一,主要成分包括NaCl、KCl、KH2PO4、Na2HPO4、NaHCO3、CaCl2、MgCl2、MgSO4和葡萄糖。Hank's平衡鹽溶液常用于組織塊的漂洗、細(xì)胞的洗滌、細(xì)胞或組織的運(yùn)輸、其他試劑的配制以及作為細(xì)胞計(jì)數(shù)的稀釋液等。
產(chǎn)品信息
形態(tài) | 液體 |
濃度 | 1× |
規(guī)格 | 500mL |
pH | 7.0-7.4 |
CaCl2(無水) | 140mg/L |
MgCl2?6H2O | 100mg/L |
MgSO4?7H2O | 100mg/L |
NaHCO3 | 350mg/L |
D-葡萄糖 | 1000mg/L |
酚紅指示劑 | 無 |
儲(chǔ)存條件 | 2-30℃ |
運(yùn)輸條件 | 室溫 |
有效期 | 36個(gè)月 |
注意事項(xiàng)
1、本產(chǎn)品為即用型,經(jīng)0.1μm過濾除菌,可直接使用,使用前無需稀釋或過濾除菌等任何處理;
2、使用本產(chǎn)品時(shí)應(yīng)注意無菌操作,避免污染;
3、由于Hank's平衡鹽溶液含有鈣鎂離子,易產(chǎn)生少量沉淀,一般溫浴后沉淀即可溶解;若沉淀較多且又無法溶解時(shí),請(qǐng)放棄使用;
4、由于Hank's平衡鹽溶液NaHCO3含量較低,不能用于5% CO2的環(huán)境,若放入CO2培養(yǎng)箱,溶液將迅速變酸,使用時(shí)應(yīng)注意;
5、本產(chǎn)品僅用于科研或進(jìn)一步研究使用,不用于診斷和治療。
參考文獻(xiàn)
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