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FasL/TNFSF6/CD95L/CD178, Human, Recombinant
貨號：PCK291
價格：￥980￥￥￥
規(guī)格：
10μg
- 10μg
產(chǎn)品概述
產(chǎn)品信息
| 別名 | soluble Fas Ligand (sFasL), TNFSF6, CD95L, Apo I Ligand, APTL, APT1LG1, CD178 |
| 物種 | Human |
| 表達(dá)宿主 | E.coli |
| 序列信息 | QIGHPSPPPEKKELRKVAHLTGKSNSRSMPLEWEDTYGIVLLSGVKYKKGGLVINETGLYFVYSKVYFRGQSCNNLPLSHKVYMRNSKYPQDLVMMEGKMMSYCTTGQMWARSSYLGAVFNLTSADHLYVNVSELSLVNFEESQTFFGLYKL with polyhistidine tag and sumo tag at the N- terminus |
| 檢索號 | P48023.1 |
| 分子量 | 17.31 kDa |
| 表觀分子量 | 30-35 kDa |
| 標(biāo)簽 | His-SUMO tag at the N-terminus |
| 生物活性 | Measure by its ability to induce apoptosis in Jurkat cells.The ED50 for this effect is <1 ng/mL. The specific activity of recombinant human FasL is > 1 x 10^6 IU/mg. |
產(chǎn)品特性
| 純度 | >98% as determined by SDS-PAGE. Ni-NTA chromatography |
| 內(nèi)毒素 | <0.1 EU per 1 μg of the protein by the LAL method. |
| 保存 | Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1%BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months. |
| 運(yùn)輸 | Ambient temperature or ice pack. |
| 制劑 | The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 8.0. |
| 復(fù)融 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein. |
背景介紹
FasL is a member of the TNF superfamily, and is mainly expressed on the cell surface of activated T cells. FasL induces apoptosis in Fas-bearing cells by binding to Fas Receptor. FasL has the ability to leads to down-regulation of the immune response through killing T cells and activated B cells. The mechanism of Fas-induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD, followed by processing of the pro-enzyme into active forms. These active caspases then cleave various cellular substrates, leading to the eventual cell death.
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