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CD178, Mouse, Recombinant
一鍵復(fù)制產(chǎn)品信息

貨號(hào)：PCK330

價(jià)格：￥980￥￥￥

規(guī)格：

10μg

10μg

數(shù)量： - +

說(shuō)明書(shū)

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產(chǎn)品概述

產(chǎn)品信息

別名	soluble Fas Ligand (sFasL), TNFSF6, CD95L, Apo I Ligand, APTL, APT1LG1, CD178, Fas-Lg, Tnfs, Tnlg1a, gld
物種	Mouse
表達(dá)宿主	E.coli
序列信息	QIANPSTPSEKKEPRSVAHLTGNPHSRSIPLEWEDTYGTALISGVKYKKGGLVINETGLYFVYSKVYFRGQSCNNQPLNHKVYMRNSKYPEDLVLMEEKRLNYCTTGQIWAHSSYLGAVFNLTSADHLYVNISQLSLINFEESKTFFGLYKL with polyhistidine tag at the N-terminus.
檢索號(hào)	P41047.1
分子量	18.27 kDa
表觀分子量	17-25 kDa
標(biāo)簽	His-tag?at?the?N-terminus
生物活性	Measure by its ability to induce apoptosis in Jurkat cells.The ED50 for this effect is <1 μg /mL.

產(chǎn)品特性

純度	>98% as determined by SDS-PAGE. Ni-NTA chromatography
內(nèi)毒素	<0.1 EU per 1 μg of the protein by the LAL method.
保存	Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1%BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months.
運(yùn)輸	Ambient temperature or ice pack.
制劑	The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 8.0.
復(fù)融	It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

背景介紹

FasL is a member of the TNF superfamily, and is mainly expressed on the cell surface of activated T cells. FasL induces apoptosis in Fas-bearing cells by binding to Fas Receptor. FasL has the ability to leads to down-regulation of the immune response through killing T cells and activated B cells. The mechanism of Fas-induced apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD, followed by processing of the pro-enzyme into active forms. These active caspases then cleave various cellular substrates, leading to the eventual cell death.