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HEPES液(1 M)
貨號(hào):PB180325
價(jià)格:¥200¥¥¥
- 100mL
產(chǎn)品概述
HEPES全稱為N-2-Hydroxyethylpiperazine-N-2-Ethane Sulfonic Acid,中文名稱為4-羥乙基哌嗪乙磺酸,是一種常用的生物緩沖劑,pKa (25℃)=7.5,有效緩沖范圍為pH 6.8-8.2。HEPES最大的優(yōu)點(diǎn)是,能夠在開(kāi)放環(huán)境中較長(zhǎng)時(shí)間恒定溶液的PH范圍。HEPES不能通過(guò)生物膜,對(duì)生化反應(yīng)影響有限,是一種化學(xué)和酶穩(wěn)定的物質(zhì),而且HEPES對(duì)可見(jiàn)光和紫外光的吸收度極低。因此一般認(rèn)為,HEPES是一種優(yōu)良的生物緩沖劑,對(duì)細(xì)胞無(wú)毒性作用,使用終濃度為10-50 mM,細(xì)胞培養(yǎng)常用10 mM。
產(chǎn)品信息
形態(tài) | 液體 |
濃度 | 1 M |
規(guī)格 | 100mL |
PH | 7.2-7.4 |
溶劑 | 純化水 |
HEPES | 238.3 g/L |
儲(chǔ)存條件 | 2-8℃或-5~-20℃,避光。 |
運(yùn)輸條件 | 常溫 |
有效期 | 2-8℃,24個(gè)月;-5~-20℃,長(zhǎng)期。 |
注意事項(xiàng)
1、本產(chǎn)品經(jīng)0.1 μm過(guò)濾除菌;
2、使用本產(chǎn)品時(shí)應(yīng)注意無(wú)菌操作,避免污染;
3、使用后請(qǐng)及時(shí)避光保存,有研究報(bào)道培養(yǎng)液中的HEPES在可見(jiàn)光下暴露3h以上將產(chǎn)生對(duì)細(xì)胞有毒性的過(guò)氧化氫;
4、本產(chǎn)品僅用于科研或進(jìn)一步研究使用,不用于診斷和治療。
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