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產品概述
MTT全稱為3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide,漢語化學名為 3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴鹽,商品名:噻唑藍,是一種黃顏色的染料。MTT能夠檢測細胞存活和生長,其檢測原理為:活細胞線粒體中的琥珀酸脫氫酶,能使外源性MTT還原為不溶于水的藍紫色結晶物--甲瓚(Formazan),并沉積在細胞中,而死細胞則無此功能;二甲基亞砜(DMSO)能溶解細胞中的甲瓚,用酶標儀在490nm或570nm波長處(常用490nm)測定其光吸收值(OD值),通過OD值的大小,可以判斷活細胞的數(shù)量或活性,在一定細胞數(shù)范圍內,MTT結晶形成的量與活細胞的數(shù)量或活性成正比。該方法的特點是靈敏度高,并且十分經(jīng)濟,已廣泛應用于一些生物活性因子的活性檢測、大規(guī)模的抗腫瘤藥物篩選、細胞毒性試驗以及腫瘤放射敏感性測定等。
產品信息
形態(tài) | 液體 |
規(guī)格 | 1mL×5/1mL×10 |
PH | 7.2~7.4 |
MTT濃度 | 5mg/mL |
溶劑 | 10mM PBS緩沖液 |
儲存條件 | -5~-20℃,避光 |
運輸條件 | 冷凍 |
有效期 | 至少18個月 |
注意事項
1、本產品經(jīng)0.22μm過濾除菌,使用本產品時應注意無菌操作,避免污染。
2、MTT溶液為黃色溶液,見光易分解,操作時應避光,當MTT溶液顏色變?yōu)榛揖G色時,請勿使用。
3、MTT溶液在4℃、冰浴等較低溫度情況下會凝固,可以在20℃~25℃水浴中溫育片刻至全部融解混勻后使用。
4、使用MTT時應先混勻,不宜長時間放置于室溫環(huán)境;2~8℃避光保存時,一個月內有效;切忌反復凍融,用量較少時建議分裝凍存。
5、MTT對菌很敏感,培養(yǎng)細胞時盡量細菌避免污染。
6、MTT有致癌性,應做好防護工作。
7、本產品僅用于科研或進一步研究使用,不用于診斷和治療。
產品手冊
參考文獻
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