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產(chǎn)品概述
M199全稱Medium 199,即培養(yǎng)基199,是Morgan等在1950年設(shè)計的,最初用于雞胚成纖維細胞的營養(yǎng)研究,現(xiàn)已廣泛應(yīng)用于各種動物細胞的培養(yǎng),包括一些非哺乳類動物細胞。M199特別適用于非轉(zhuǎn)化細胞的培養(yǎng),也常用于病毒學、疫苗生產(chǎn),以及大鼠胰腺上皮細胞和小鼠晶狀體組織的培養(yǎng)。與其他基礎(chǔ)培養(yǎng)基相比,M199含有獨特的成分,包括腺嘌呤、腺苷、次黃嘌呤、胸腺嘧啶、以及其他的維生素。M199有兩種平衡鹽成分,Earle's鹽成分常用于CO2環(huán)境,Hank's鹽成分用于非CO2的環(huán)境。
本產(chǎn)品含有多類細胞培養(yǎng)所需的氨基酸、維生素、無機鹽等多種成分,但不含蛋白質(zhì)、脂類或任何生長因子,故此產(chǎn)品需搭配血清或無血清添加物使用。
成分說明
形態(tài) | 液體 |
濃度 | 1× |
規(guī)格 | 500mL |
PH | 7.2~7.4 |
平衡鹽 | Earle's 鹽 |
L-谷氨酰胺 | 100mg/L |
NaHCO3 | 2200mg/L |
D-葡萄糖 | 1000mg/L |
HEPES緩沖劑 | 無 |
酚紅指示劑 | 20mg/L |
儲存條件 | 2~8℃,避光 |
運輸條件 | 常溫 |
有效期 | 18個月 |
注意事項
1、本產(chǎn)品經(jīng)過濾除菌,使用時應(yīng)注意無菌操作,避免污染;
2、為保持本產(chǎn)品的最佳使用效果,請勿進行凍融處理;
3、本產(chǎn)品僅用于科研或進一步研究使用,不用于診斷和治療。
參考文獻
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DOI:10.1007/s00253-023-12433-3
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作者:Lanfei Xiao, Chuan Chen, Yongjun Liang
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DOI:10.1016/j.aquaculture.2024.740593
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