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人椎間盤髓核細(xì)胞
Human Intervertebral Disc Nucleus Pulposu Cells
貨號：CP-H097
規(guī)格：5×10?Cells/T25培養(yǎng)瓶
價(jià)格： ￥詢價(jià)
產(chǎn)品概述
人椎間盤髓核細(xì)胞
Cat NO.: CP-H097
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產(chǎn)品名稱：人椎間盤髓核細(xì)胞
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英文簡稱：NPCs
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組織來源：椎間盤組織
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產(chǎn)品規(guī)格：5×105cells/T25細(xì)胞培養(yǎng)瓶
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細(xì)胞簡介：
人椎間盤髓核細(xì)胞分離椎間盤組織；椎間盤是位于脊柱兩椎體之間，分為中央部的髓核，富于彈性的膠狀物質(zhì)；周圍部的纖維環(huán)，由多層纖維軟骨環(huán)按同心圓排列。上下有軟骨板，是透明軟骨復(fù)蓋于椎體上，下面骺環(huán)中間的骨面。上下的軟骨板與纖維環(huán)一起將髓核密封起來。纖維環(huán)由膠原纖維束的纖維軟骨構(gòu)成，位于髓核的四周。纖維環(huán)的纖維束相互斜行交叉重疊，使纖維環(huán)成為堅(jiān)實(shí)的組織，能承受較大的彎曲和扭轉(zhuǎn)負(fù)荷。髓核，是乳白色半透明膠狀體，富于彈性，為椎間盤結(jié)構(gòu)的一部分，位于兩軟骨板與纖維環(huán)之間。由縱橫交錯的纖維網(wǎng)狀結(jié)構(gòu)即軟骨細(xì)胞和蛋白多糖黏液樣基質(zhì)構(gòu)成的彈性膠凍物質(zhì)。嬰幼兒時(shí)期的髓核含水量為80%-90%，即使到了老年，其含水量也在70%上下。髓核在出生時(shí)體積大而松散，位于椎間盤的中央，至成年時(shí)位置移至椎間盤的中后部；在成年以前構(gòu)成髓核的主要物質(zhì)是大量蛋白多糖復(fù)合體、膠原纖維和纖維軟骨，隨著年齡的增長，髓核中的蛋白多糖解聚增多，水分逐漸減少，膠原增粗并逐漸被纖維軟骨所替代。
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方法簡介：
普諾賽實(shí)驗(yàn)室分離的人椎間盤髓核細(xì)胞采用膠原酶-中性蛋白酶混合消化法并結(jié)合軟骨細(xì)胞專用培養(yǎng)基培養(yǎng)篩選制備而來，細(xì)胞總量約為5×10?cells/瓶。
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質(zhì)量檢測：
普諾賽實(shí)驗(yàn)室分離的人椎間盤髓核細(xì)胞經(jīng)Ⅱ型膠原蛋白免疫熒光鑒定，純度可達(dá)90%以上，且不含有HIV-1、HBV、HCV、支原體、細(xì)菌、酵母和真菌等。
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培養(yǎng)信息：
包被條件 培養(yǎng)基 含F(xiàn)BS、生長添加劑、Penicillin、Streptomycin等 產(chǎn)品貨號 CM-H097 換液頻率 每3-4天換液一次 生長特性 貼壁 細(xì)胞形態(tài) 梭形、多角形 傳代特性 可傳5代左右；3代以內(nèi)狀態(tài)最佳 消化液 0.25%胰蛋白酶 培養(yǎng)條件 氣相：空氣，95%；CO2，5% 人椎間盤髓核細(xì)胞體外培養(yǎng)周期有限；建議使用普諾賽配套的專用生長培養(yǎng)基及正確的操作方法來培養(yǎng)，以此保證該細(xì)胞的最佳培養(yǎng)狀態(tài)。
產(chǎn)品手冊
參考文獻(xiàn)
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Pharmacological network analysis of the functions and mechanism of kaempferol from Du Zhong in intervertebral disc degeneration (IDD)(2023/03/03)
作者：Xiaobin Wang, Yanlin Tan, Fusheng Liu
期刊：Journal of Orthopaedic Translation
影響因子 ：6.600
引用產(chǎn)品：CP-H097
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hASCs-derived exosomal miR-155-5p targeting TGFβR2 promotes autophagy and reduces pyroptosis to alleviate intervertebral disc degeneration(2023/03/14)
作者：Dong Chen, Xin Jiang, Haibo Zou
期刊：Journal of Orthopaedic Translation
影響因子 ：6.600
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BSHXF-medicated serum combined with ADSCs regulates the TGF-β1/Smad pathway to repair oxidatively damaged NPCs and its component analysis(2023/06/03)
作者：Jiahao Duan, Zhaoyong Li, Enxu Liu
期刊：JOURNAL OF ETHNOPHARMACOLOGY
影響因子 ：5.400
引用產(chǎn)品：CP-H097
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Exogenous Klotho ameliorates extracellular matrix degradation and angiogenesis in intervertebral disc degeneration via inhibition of the Rac1/PAK1/MMP-2 signaling axis(2022/08/08)
作者：Yuyang Yi, Hao Chen, Shubao Zhang
期刊：MECHANISMS OF AGEING AND DEVELOPMENT
影響因子 ：5.300
引用產(chǎn)品：CP-H097
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microRNA-365 attenuated intervertebral disc degeneration through modulating nucleus pulposus cell apoptosis and extracellular matrix degradation by targeting EFNA3(2023/11/27)
作者：Chao Jiang, Youjun Liu, Weigong Zhao
期刊：JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
影響因子 ：5.300
引用產(chǎn)品：CP-H097
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Lumican silencing alleviates tumor necrosis factor-α-induced nucleus pulposus cell inflammation and senescence by inhibiting apoptosis signal regulating kinase 1/p38 signaling pathway via inactivating Fas ligand expression(2021/9/13)
作者：Zhenqiang Li, Chengfeng Sun, Maosong Chen
期刊：Bioengineered
DOI：10.1080/21655979.2021.1973781
影響因子 ：4.900
引用產(chǎn)品：CP-H097
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The protective effects of dezocine on interleukin-1β-induced inflammation, oxidative stress and apoptosis of human nucleus pulposus cells and the possible mechanisms(2022/01/03)
作者：Fang Zhu, Wei Duan, Chao Zhong
期刊：Bioengineered
DOI：10.1080/21655979.2021.2017700
影響因子 ：4.900
引用產(chǎn)品：CP-H097
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Kukoamine A attenuates lipopolysaccharide-induced apoptosis, extracellular matrix degradation, and inflammation in nucleus pulposus cells by activating the P13K/Akt pathway(2022/03/25)
作者：Dan Wang?,?Hao Qu?,?Hui Kang
期刊：Bioengineered
DOI：10.1080/21655979.2022.2051855
影響因子 ：4.900
引用產(chǎn)品：CP-H097
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MicroRNA-137 inhibits the inflammatory response and extracellular matrix degradation in lipopolysaccharide-stimulated human nucleus pulposus cells by targeting activin a receptor type I(2022/03/02)
作者：Bin Yu, Ziqi Zhu, Beiduo Shen
期刊：Bioengineered
DOI：10.1080/21655979.2022.2042987
影響因子 ：4.900
引用產(chǎn)品：CP-H097
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Platelet-rich plasma-derived extracellular vesicles inhibit NF-κB/NLRP3 pathway-mediated pyroptosis in intervertebral disc degeneration via the MALAT1/microRNA-217/SIRT1 axis(2024/02/17)
作者：Xueqiang Tao, Fen Xue, Jiayuan Xu
期刊：CELLULAR SIGNALLING
DOI：10.1016/j.cellsig.2024.111106
影響因子 ：4.800
引用產(chǎn)品：CP-H097
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Micro Fragmented Adipose Tissue Promotes the Matrix Synthesis Function of Nucleus Pulposus Cells and Regenerates Degenerated Intervertebral Disc in a Pig Model:(2020/2/7)
作者：Xiaopeng Zhou, Feng Zhang, Dawei Wang
期刊：Cell Transplantation
影響因子 ：3.300
引用產(chǎn)品：CP-H097
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miR-4478 Accelerates Nucleus Pulposus Cells Apoptosis Induced by Oxidative Stress by Targeting MTH1(2022/09/20)
作者：Jiafang Zhang, Ruiduan Liu, Ling Mo
期刊：SPINE
DOI：10.1097/BRS.0000000000004486
影響因子 ：3.241
引用產(chǎn)品：CP-H097
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Retinoic acid inhibits the pyroptosis of degenerated nucleus pulposus cells by activating Sirt1-SOD2 signaling(2023/04/02)
作者：Pengfei Li, Fei Xiong, Hongyuan Xing
期刊：CONNECTIVE TISSUE RESEARCH
DOI：10.1080/03008207.2023.2192286
影響因子 ：2.900
引用產(chǎn)品：CP-H097
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Overloaded axial stress activates the Wnt/β-Catenin pathway in nucleus pulposus cells of adult degenerative scoliosis combined with intervertebral disc degeneration(2023/04/08)
作者：Zhijun Cai, Qibiao Luo, Xi Yang
期刊：MOLECULAR BIOLOGY REPORTS
DOI：10.1007/s11033-023-08390-9
影響因子 ：2.800
引用產(chǎn)品：CP-H097
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Role of lncRNA PART1 in intervertebral disc degeneration and associated underlying mechanism(2020/12/10)
作者：Zongyu Zhang, Yongfeng Huo, Zhijing Zhou
期刊：Experimental and Therapeutic Medicine
影響因子 ：2.700
引用產(chǎn)品：CP-H097
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Dimethyl fumarate protects nucleus pulposus cells from inflammation and oxidative stress and delays the intervertebral disc degeneration(2020/10/27)
作者：Hainian Zhu, Gang Chen, Yuhua Wang
期刊：Experimental and Therapeutic Medicine
影響因子 ：2.700
引用產(chǎn)品：CP-H097
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miR?489?3p overexpression inhibits lipopolysaccharide?induced nucleus pulposus cell apoptosis, inflammation and extracellular matrix degradation via targeting Toll?like receptor 4(2021/9/20)
作者：Ling Dong, Bo Dong
期刊：Experimental and Therapeutic Medicine
影響因子 ：2.700
引用產(chǎn)品：CP-H097
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Experimental study of miR-503 regulating the activity as well as the function of degenerated human nucleus pulposus cells of the intervertebral disc through inhibiting Wnt pathway.(2023/03/01)
作者：Xiaoming Shi, Shaohua Tian, Yinan Tian
期刊：JOURNAL OF MUSCULOSKELETAL & NEURONAL INTERACTIONS
DOI：PMID:36856108
影響因子 ：1.900
引用產(chǎn)品：CP-H097
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Mesenchymal stem cell-derived exosomes are beneficial to suppressing inflammation and promoting autophagy in intervertebral disc degeneration(2023/03/22)
作者：Baicheng Yang, Xinming Yang
期刊：FOLIA MORPHOLOGICA
影響因子 ：1.200
引用產(chǎn)品：CP-H097
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CircRNA RERE Promotes the Oxidative Stress-Induced Apoptosis and Autophagy of Nucleus Pulposus Cells through the miR-299-5p/Galectin-3 Axis(2021/12/15)
作者：Rong Wang, Xingchao Zhou, Guorui Luo
期刊：Journal of Healthcare Engineering
影響因子 ：0.000
引用產(chǎn)品：CP-H097
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