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人軟骨細(xì)胞
Human Chondrocyte Cells
貨號:CP-H107
規(guī)格:5×10?Cells/T25培養(yǎng)瓶
價格: ¥6380
產(chǎn)品概述
人軟骨細(xì)胞
Cat NO.: CP-H107
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產(chǎn)品名稱:人軟骨細(xì)胞
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組織來源:軟骨組織
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產(chǎn)品規(guī)格:5×105cells/T25細(xì)胞培養(yǎng)瓶
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細(xì)胞簡介:
人軟骨細(xì)胞分離自軟骨組織;軟骨組織由軟骨細(xì)胞、基質(zhì)及纖維構(gòu)成。軟骨組織再生能力強(qiáng),這些增生的幼稚細(xì)胞形似纖維母細(xì)胞,以后逐漸變?yōu)檐浌悄讣?xì)胞,并形成軟骨基質(zhì),細(xì)胞被埋在軟骨陷窩內(nèi)而變?yōu)殪o止的軟骨細(xì)胞。根據(jù)軟骨組織內(nèi)所含纖維成分的不同,可將軟骨分為透明軟骨、彈性軟骨和纖維軟骨三種,其中以透明軟骨的分布較廣,結(jié)構(gòu)也較典型。軟骨細(xì)胞(chondrocyte)位于軟骨陷窩內(nèi)。幼稚的軟骨細(xì)胞位于軟骨組織的表層,單個分布,體積較小,呈橢圓形,長軸與軟骨表面平行,越向深層的軟骨細(xì)胞體積之間增大呈圓形,細(xì)胞核圓形或卵圓形,染色淺,細(xì)胞質(zhì)弱嗜堿性,常見數(shù)量不一的脂滴。成熟的軟骨細(xì)胞多2-8個成群分布于軟骨陷窩內(nèi),這些軟骨細(xì)胞由同一個母細(xì)胞分裂增殖而成,稱為同源細(xì)胞群。電鏡下,軟骨細(xì)胞有突起和皺褶,細(xì)胞質(zhì)內(nèi)有大量的粗面內(nèi)質(zhì)網(wǎng)和發(fā)達(dá)的高爾基復(fù)合體及少量的線粒體。在組織切片中,軟骨細(xì)胞收縮為不規(guī)則形,在軟骨囊和細(xì)胞之間出現(xiàn)較大的腔隙。體外培養(yǎng)的軟骨細(xì)胞對于研究其生理功能、藥物作用以及各種致病因素作用下的病理生理改變具重要意義。
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方法簡介:
普諾賽實驗室分離的人軟骨細(xì)胞采用膠原酶 - 中性蛋白酶聯(lián)合消化制備而來,細(xì)胞總量約為5×10?cells/瓶。
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質(zhì)量檢測:
普諾賽實驗室分離的人軟骨細(xì)胞經(jīng)Ⅱ型膠原蛋白免疫熒光鑒定,純度可達(dá)90%以上,且不含有HIV-1、HBV、HCV、支原體、細(xì)菌、酵母和真菌等。
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培養(yǎng)信息:
包被條件 培養(yǎng)基 含F(xiàn)BS、生長添加劑、Penicillin、Streptomycin等 產(chǎn)品貨號 CM-H107 換液頻率 每2-3天換液一次 生長特性 貼壁 細(xì)胞形態(tài) 梭形、多角形 傳代特性 可傳5代左右;3代以內(nèi)狀態(tài)最佳 消化液 0.25%胰蛋白酶 培養(yǎng)條件 氣相:空氣,95%;CO2,5% 人軟骨細(xì)胞體外培養(yǎng)周期有限;建議使用普諾賽配套的專用生長培養(yǎng)基及正確的操作方法來培養(yǎng),以此保證該細(xì)胞的最佳培養(yǎng)狀態(tài)。
產(chǎn)品手冊
參考文獻(xiàn)
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Lubricating Micro-Interface Assisted General Strategy for Preparing dECM-Microparticle–Based Heterogeneous Granular Inks toward 3D Printing(2023/12/11)
作者:Kunxi Zhang, Yinan Zhu, Tao Wang
期刊:ADVANCED FUNCTIONAL MATERIALS
影響因子 :19.000
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Fabrication of chondroitin sulfate calcium complex and its chondrocyte proliferation in vitro(2020/10/22)
作者:Qingshan Shen, Chunhui Zhang, Haizhen Mo
期刊:Carbohydrate Polymers
DOI:10.1016/j.carbpol.2020.117282
影響因子 :11.200
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HWJMSC-EVs promote cartilage regeneration and repair via the ITGB1/TGF-β/Smad2/3 axis mediated by microfractures(2024/04/12)
作者:Zhian Chen, Tianhua Zhou, Huan Luo
期刊:JOURNAL OF NANOBIOTECHNOLOGY
DOI:10.1186/s12951-024-02451-2
影響因子 :10.200
引用產(chǎn)品:CP-H107
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Plasma exosome miRNA-26b-3p derived from idiopathic short stature impairs longitudinal bone growth via the AKAP2/ERK1/2 axis(2023/03/16)
作者:Xijuan Liu, Jinghong Yuan, Zhiwen Wu
期刊:JOURNAL OF NANOBIOTECHNOLOGY
DOI:10.1186/s12951-023-01849-8
影響因子 :10.200
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Circ_SPG11 plays contributing effects on IL-1β-induced chondrocyte apoptosis and ECM degradation via miR-665 inhibition-mediated GREM1 upregulation(2021/11/17)
作者:Xiao Ouyang, Yunzhi Ding, Li Yu
期刊:Clinical Immunology
DOI:10.1016/j.clim.2021.108889
影響因子 :8.600
引用產(chǎn)品:CP-H107
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Silver nanoparticles inhibit the function of hypoxia-inducible factor-1 and target genes: insight into the cytotoxicity and antiangiogenesis(2016/12/08)
作者:Tieshan Yang, Qian Yao, Fei Cao
期刊:International Journal of Nanomedicine
影響因子 :8.000
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Activation of microglial GLP-1R in the trigeminal nucleus caudalis suppresses central sensitization of chronic migraine after recurrent nitroglycerin stimulation(2021/07/29)
作者:Feng Jing, Qian Zou, Yangyang Wang
期刊:JOURNAL OF HEADACHE AND PAIN
DOI:10.1186/s10194-021-01302-x
影響因子 :7.400
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Circular RNA circANAPC2 mediates the impairment of endochondral ossification by miR‐874‐3p/SMAD3 signalling pathway in idiopathic short stature(2021/03/13)
作者:Xijuan Liu, Zhi Du, Xuan Yi
期刊:Journal Of Cellular And Molecular Medicine
影響因子 :5.300
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Hsa_circ_0134111 promotes osteoarthritis progression by regulating miR-224-5p/CCL1 interaction(2021/08/19)
作者:Yongbao Liu, Yanxiu Zhang
期刊:Aging-US
影響因子 :5.200
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A Novel Diagnostic Predictive Model for Idiopathic Short Stature in Children(2021/09/07)
作者:Jinghong Yuan, Zhi Du, Zhiwen Wu
期刊:Frontiers in Endocrinology
影響因子 :5.200
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Valproic Acid Protects Chondrocytes from LPS-Stimulated Damage via Regulating miR-302d-3p/ITGB4 Axis and Mediating the PI3K-AKT Signaling Pathway(2021/04/22)
作者:Long Sun, Wei Zheng, Qiandong Liu
期刊:Frontiers in Molecular Biosciences
影響因子 :5.000
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Hsa_circ_0005567 Activates Autophagy and Suppresses IL-1β-Induced Chondrocyte Apoptosis by Regulating miR-495(2020/08/25)
作者:Jinling Zhang, Fangyue Cheng, Genxiang Rong
期刊:Frontiers in Molecular Biosciences
影響因子 :5.000
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Downregulation of miR-3680–3p inhibits the progression of osteoarthritis via targeting OGG1(2022/01/21)
作者:Yanping Xie, Jianwei Li, Yanhui Suo
期刊:Archives of Gerontology and Geriatrics
DOI:10.1016/j.archger.2022.104626
影響因子 :4.000
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miR-590-5p affects chondrocyte proliferation, apoptosis, and inflammation by targeting FGF18 in osteoarthritis(2021/08/15)
作者:Peng Jiang,?Xixi Dou,?Shuaiguang Li
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DOI:PMID:34539990
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Regulation of circADAMTS6-miR-324-5p-PIK3R3 ceRNA pathway may be a novel mechanism of IL-1β-induced osteoarthritic chondrocytes(2022/03/25)
作者:Lanjuan Shen, Cheng Ji, Jian Lin
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DOI:10.1007/s00774-021-01308-0
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Circ_0136474 promotes the progression of osteoarthritis by sponging mir-140-3p and upregulating MECP2(2022/11/26)
作者:Cheng, Shile, Nie, Zhigang, Cao, Jiarui, Peng, Hao
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Inhibition of miR-182-5p Targets FGF9 to Alleviate Osteoarthritis(2023/03/29)
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Prognostic-related genes for pancreatic cancer typing and immunotherapy response prediction based on single-cell sequencing data and bulk sequencing data(2023/07/21)
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Downregulation of lncRNA NEAT1 interacts with miR-374b-5p/PGAP1 axis to aggravate the development of osteoarthritis(2023/09/11)
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DOI:10.1186/s13018-023-04147-z
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Downregulation of miR-1-3p expression inhibits the hypertrophy and mineralization of chondrocytes in DDH(2021/8/18)
作者:Ding, Rui, Liu, Xijuan, Zhang, Jian
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DOI:10.1186/s13018-021-02666-1
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Ferroptosis-related genes LPCAT3 and PGD are potential diagnostic biomarkers for osteoarthritis(2023/09/19)
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DOI:10.1186/s13018-023-04128-2
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Chondrocyte suppression is mediated by miR‐129‐5p via GDF11/SMAD3 signaling in developmental dysplasia of the hip(2020/05/12)
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Knocking-down long non-coding RNA LINC01094 prohibits chondrocyte apoptosis via regulating microRNA-577/metal-regulatory transcription factor 1 axis(2024/05/17)
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