當(dāng)前位置：首頁(yè) >> 產(chǎn)品中心 >> 輔助試劑 >> 細(xì)胞因子 >> Human

產(chǎn)品中心Cell Resources

聯(lián)系我們CONTACT US

400-999-210024小時(shí)服務(wù)熱線
sales@procell.com.cn銷(xiāo)售郵箱

LIF, Human, Recombinant
一鍵復(fù)制產(chǎn)品信息

貨號(hào)：PCK074

價(jià)格：￥1380￥3680￥17980￥27980

規(guī)格：

10μg

10μg
50μg
500μg
1mg

數(shù)量： - +

說(shuō)明書(shū)

在線咨詢

產(chǎn)品概述

產(chǎn)品信息

別名	Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor (MLP_x0002_LI), Interleukin 6 family cytokine
物種	Human
表達(dá)宿主	E.coli
序列信息	SPLPITPVNATCAIRHPCHNNLMNQIRSQLAQLNGSANALFILYYTAQGEPFPNNLDKLCGPNVTDFPPFHANGTEKAKLVELYRIVVYLGTSLGNITRDQKILNPSALSLHSKLNATADILRGLLSNVLCRLCSKYHVGHVDVTYGPDTSGKDVFQKKKLGCQLLGKYKQIIAVLAQAF with polyhistidine tag at the N-terminus.
檢索號(hào)	P15018.1
分子量	20.52 kDa
表觀分子量	18 kDa
標(biāo)簽	His-tag?at?the?N-terminus
生物活性	Measure by its ability to induce TF-1 cells proliferation.The ED50 for this effect is <0.2 ng/mL.

產(chǎn)品特性

純度	>98% as determined by SDS-PAGE. Ni-NTA chromatography
內(nèi)毒素	<0.1 EU per 1 μg of the protein by the LAL method.
保存	Lyophilized protein should be stored at -5~-20°C for 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g.0.1% BSA,10%FBS,5%HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20°C or -80°C for 3-6 months.
運(yùn)輸	Ambient temperature or ice pack.
制劑	The protein was lyophilized from a 0.2 μm filtered solution containing 1X PBS, pH 7.4.
復(fù)融	It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL.Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.

背景介紹

LIF, a pleiotrophic factor, is identified in multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma. LIF is capable of promoting long-term maintenance of embryonic stem cells by inhibiting spontaneous differentiation. In addition, LIF also have abilities including stimulation of differentiation of cholinergic nerves, the stimulation of acute phase protein synthesis by hepatocytes, and suppression of adipogenesis by supressing the lipoprotein lipase in adipocytes.