產(chǎn)品中心Cell Resources
聯(lián)系我們CONTACT US
- 400-999-210024小時(shí)服務(wù)熱線
NK-92 (人惡性非霍奇金淋巴瘤患者的自然殺傷細(xì)胞) (STR鑒定正確)
貨號:CL-0530
規(guī)格:1×10?cells/T25培養(yǎng)瓶
價(jià)格: ¥2200
生長培養(yǎng)基:MEMα+0.2mM Inositol+0.1mM β-mercaptoethanol+0.02mM Folic Acid+100-200U/mL recombinant IL-2+12.5% HS+12.5% FBS+1% P/S
產(chǎn)品概述
名稱 | NK-92 (人惡性非霍奇金淋巴瘤患者的自然殺傷細(xì)胞) (STR鑒定正確) |
別稱 | NK92; Natural Killer-92; NK-92.05; Neukoplast; aNK |
種屬 | 人類 |
生長特性 | 懸浮細(xì)胞 |
細(xì)胞形態(tài) | 淋巴母細(xì)胞樣 |
凍存條件 | 凍存液:90%FBS+10%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認(rèn)) |
生長培養(yǎng)基:
MEMα+0.2mM Inositol+0.1mM β-mercaptoethanol+0.02mM Folic Acid+100-200U/mL recombinant IL-2+12.5% HS+12.5% FBS+1% P/S
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 5×10^5-1×10^6cells/mL |
推薦換液頻率 | 2-3次/周 |
注意事項(xiàng) | 該細(xì)胞為懸浮細(xì)胞,請注意離心收集細(xì)胞懸液;請勿直接倒掉細(xì)胞培養(yǎng)液。 |
背景描述 | NK-92細(xì)胞是從一位患有急進(jìn)性非霍奇金淋巴瘤的50歲白人男性外周血單核細(xì)胞衍生來的一株IL-2依賴型NK細(xì)胞株。NK-92細(xì)胞對很多惡性細(xì)胞有細(xì)胞毒性;鉻釋放試驗(yàn)顯示它能殺死K562細(xì)胞和Daudi細(xì)胞。NK-92細(xì)胞(經(jīng)過照射以防止增殖)可以有效地用于血液中白血病的體外免疫清掃而不危及血細(xì)胞的功能。NK-92細(xì)胞有以下特征:CD2、CD7、CD11a、CD28、CD45、CD54表面標(biāo)記陽性;CD1、CD3、CD4、CD5、CD8、CD10、CD14、CD16、CD19、CD20、CD23、CD34和HLA-DR表面標(biāo)記陰性。 |
年齡(性別) | 男性;50歲 |
組織來源 | 外周血 |
細(xì)胞類型 | 腫瘤細(xì)胞 |
腫瘤類型 | 淋巴瘤細(xì)胞 |
生物安全等級 | BSL-2 |
倍增時(shí)間 | ~40-50 hours |
保藏機(jī)構(gòu) | ATCC; CRL-2407 DSMZ; ACC-488 |
STR鑒定
-
STR位點(diǎn)信息
Amelogenin X,Y CSF1PO 11,12 D2S1338 19,20 D3S1358 15 D5S818 12,13 D7S820 10,11 D8S1179 12 D13S317 9,12 D16S539 11,12 D18S51 12,17 D19S433 14,15 D21S11 31.2,32 FGA 20,22 PentaD 10,12 PentaE 12 TH01 6,9.3 TPOX 8 vWA 18 D6S1043 11,12 D12S391 19 D2S441 14 -
STR鑒定圖
參考文獻(xiàn)
-
L-kynurenine induces NK cell loss in gastric cancer microenvironment via promoting ferroptosis(2023/03/01)
作者:Jianxin Cui, Xianhui Xu, Tao He
期刊:JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
DOI:10.1186/s13046-023-02629-w
影響因子 :11.300
引用產(chǎn)品:CL-0530
-
MMP-2 Inhibitor-Mediated Tumor Microenvironment Regulation Using a Sequentially Released Bio-Nanosystem for Enhanced Cancer Photo-Immunotherapy(2022/09/08)
作者:Huifang Liu, Dongqin Lei, Jiong Li
期刊:ACS Applied Materials & Interfaces
影響因子 :9.500
引用產(chǎn)品:CL-0530
-
CD44 3-Untranslated Region Functions as a Competing Endogenous RNA to Enhance NK Sensitivity of Liver Cancer Stem Cell by Regulating ULBP2 Expression(2019/06/04)
作者:Jun Weng, Xu Han, Kaiyu Liu
期刊:International Journal of Biological Sciences
影響因子 :9.200
引用產(chǎn)品:CL-0530
-
Therapeutic effects against high-grade glioblastoma mediated by engineered induced neural stem cells combined with GD2-specific CAR-NK(2023/07/07)
作者:Weihua Liu, Yu Zhao, Zhongfeng Liu
期刊:CELLULAR ONCOLOGY
DOI:10.1007/s13402-023-00842-5
影響因子 :6.600
-
NK92 cells and peripheral blood NK cells respond oppositely upon dasatinib treatment(2024/02/15)
作者:Fengqi Li, Zhongyi Wang, Dongpeng Zheng
期刊:IMMUNOLOGY
影響因子 :6.400
引用產(chǎn)品:CL-0530
-
Enhanced NK cell activation via eEF2K-mediated potentiation of the cGAS–STING pathway in hepatocellular carcinoma(2024/02/05)
作者:Yan Xu, Fang Sun, Yuying Tian
期刊:INTERNATIONAL IMMUNOPHARMACOLOGY
DOI:10.1016/j.intimp.2024.111628
影響因子 :5.600
引用產(chǎn)品:CL-0530
-
Assessing immune hepatotoxicity of troglitazone with a versatile liver-immune-microphysiological-system(2024/05/30)
作者:Quanfeng Deng, Youlong Yang, Yuangui Liu
期刊:Frontiers in Pharmacology
DOI:10.3389/fphar.2024.1335836
影響因子 :5.600
引用產(chǎn)品:CL-0530
-
Enhanced Proinflammatory Cytokine Production and Immunometabolic Impairment of NK Cells Exposed to Mycobacterium tuberculosis and Cigarette Smoke(2022/01/05)
作者:Yafei Rao, Xiaoyan Gai, Yanqing Le
期刊:Frontiers in Cellular and Infection Microbiology
影響因子 :5.500
引用產(chǎn)品:CL-0530
-
Platelet-derived major histocompatibility complex class I coating on Treponema pallidum attenuates natural killer cell lethality(2024/05/14)
作者:Qiuyan Xu, Xinqi Zheng, Weiming Ye
期刊:Virulence
DOI:10.1080/21505594.2024.2350892
影響因子 :5.200
引用產(chǎn)品:CL-0530
-
PKCι regulates the expression of PDL1 through multiple pathways to modulate immune suppression of pancreatic cancer cells(2021/8/8)
作者:Hongmei Zhang, Yue Zhu, Junli Wang
期刊:Cellular Signalling
DOI:10.1016/j.cellsig.2021.110115
影響因子 :4.800
引用產(chǎn)品:CL-0530
-
Transcription factor Sp1 is upregulated by PKCι to drive the expression of YAP1 during pancreatic carcinogenesis(2021/4/17)
作者:Jinhe Yang, Junli Wang, Hongmei Zhang
期刊:Carcinogenesis
影響因子 :4.700
引用產(chǎn)品:CL-0530
-
Development of TSHR-CAR NK-92 Cells for Differentiated Thyroid Cancer(2024/04/24)
作者:Jiahui Zhou, Chengcheng Zhang, Weibo Mao
期刊:MOLECULAR AND CELLULAR ENDOCRINOLOGY
影響因子 :4.100
引用產(chǎn)品:CL-0530
-
SHFM1 deficiency suppresses esophageal squamous cell carcinomas progression via modulating NF?κB signaling and enhancing nature killer cell?mediated tumor surveillance(2023/03/17)
作者:Yijuan Wu, Zhiyu Wang, Shengmian Li
期刊:Experimental and Therapeutic Medicine
影響因子 :2.700
FAQs
Q:{{item.question}}
A:
產(chǎn)品資料
識別碼示意圖