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產(chǎn)品概述
名稱 | OE19 (人食管癌細胞) (STR鑒定正確) |
別稱 | OE-19; JROECL 19; JROECL19; OEC19 |
種屬 | 人 |
生長特性 | 貼壁細胞 |
細胞形態(tài) | 上皮細胞樣 |
凍存條件 | 凍存液:55% 基礎培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認) |
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 1:2-1:4 |
推薦換液頻率 | 2-3天/次 |
背景描述 | OE19, also known as JROECL19, was originally one of five permanent human oesophageal adenocarcinoma (EAC) cell lines established in 1993. It is from a stage III tumour of a 72 year old male patient1 and deposited in ECACC by Drs J C Rockett/A Morris, Department of Biological Sciences, University of Warwick and Dr S J Darnton, Birmingham Heartlands Hospital. Boonstra et al. discovered that cell lines SEG-1, BIC-1, and SK-GT-5 are not EAC cell lines but large cell lung cancer cell line H460, colorectal adenocarcinoma cell line SW620, and gastric fundus carcinoma cell line SKGT-2, respectively. However, they confirmed that cell lines FLO-1, KYAE-1, SK-GT-4, OE19, OE33, OACP4C, OACM5.1, ESO26, and ESO51 are derived from human EACs2 . All nine of these verified EAC cell lines, together with their genotyping information, have been deposited with ECACC. |
年齡(性別) | 男性;72歲 |
組織來源 | 食管癌 |
細胞類型 | 腫瘤細胞 |
腫瘤類型 | 食管癌細胞 |
生物安全等級 | BSL-1 |
倍增時間 | ~50-60 hours (DSMZ=ACC-700) |
致瘤性 | 裸小鼠致癌 |
保藏機構 | ECACC; 96071721 |
STR鑒定
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STR位點信息
Amelogenin X CSF1PO 11,13 D2S1338 18,19 D3S1358 15,18 D5S818 11,14 D7S820 8 D8S1179 13,15 D13S317 9,11 D16S539 12,13 D18S51 12 D19S433 13 D21S11 30 FGA 23,26 PentaD 9 PentaE 5,8 TH01 8,9 TPOX 8 vWA 16,17,18 D6S1043 19 D12S391 20 D2S441 14,15 -
STR鑒定圖
參考文獻
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COL8A1 Regulates Esophageal Squamous Carcinoma Proliferation and Invasion Through PI3K/AKT Pathway(2024/03/01)
作者:Jing Hu, Pengbo Li, Yanggang Dan
期刊:ANNALS OF SURGICAL ONCOLOGY
DOI:10.1245/s10434-023-14370-x
影響因子 :3.700
引用產(chǎn)品:CL-0754
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