OE19(人食管癌細胞)
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英文名稱:OE19
貨號: CL-0754
價格: ¥1800
套餐組合價
產(chǎn)品信息
產(chǎn)品名稱 | OE19(人食管癌細胞) |
別稱 | OE-19;JROECL 19;JROECL19;OEC19 |
種屬 | 人 |
生長特性 | 貼壁細胞 |
細胞形態(tài) | 上皮細胞樣 |
凍存條件 |
凍存液:55% 基礎(chǔ)培養(yǎng)基+40% FBS+5% DMSO 溫度:液氮 |
培養(yǎng)方案A(默認) |
生長培養(yǎng)基:RPMI-1640+10% FBS+1% P/S 培養(yǎng)條件:氣相:空氣,95%;CO?,5%, 溫度:37℃ |
推薦傳代比例 | 1:2-1:4 |
推薦換液頻率 | 2-3天/次 |
參考資料(來源文獻)
背景描述 | OE19, also known as JROECL19, was originally one of five permanent human oesophageal adenocarcinoma (EAC) cell lines established in 1993. It is from a stage III tumour of a 72 year old male patient1 and deposited in ECACC by Drs J C Rockett/A Morris, Department of Biological Sciences, University of Warwick and Dr S J Darnton, Birmingham Heartlands Hospital. Boonstra et al. discovered that cell lines SEG-1, BIC-1, and SK-GT-5 are not EAC cell lines but large cell lung cancer cell line H460, colorectal adenocarcinoma cell line SW620, and gastric fundus carcinoma cell line SKGT-2, respectively. However, they confirmed that cell lines FLO-1, KYAE-1, SK-GT-4, OE19, OE33, OACP4C, OACM5.1, ESO26, and ESO51 are derived from human EACs2. All nine of these verified EAC cell lines, together with their genotyping information, have been deposited with ECACC. |
年齡(性別) | 72Y;Male |
組織來源 | 食管癌 |
細胞類型 | 腫瘤細胞 |
腫瘤類型 | 食管癌細胞 |
生物安全等級 | BSL-1 |
致瘤性 | 裸小鼠致癌 |
位點信息
鑒定圖
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期刊:NEOPLASIA
影響因子:6.3
引用產(chǎn)品: 青霉素-鏈霉素溶液(雙抗),100×, OE19 細胞, TE-12 細胞
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期刊:ANNALS OF SURGICAL ONCOLOGY
DOI:10.1245/s10434-023-14370-x
影響因子:3.7
引用產(chǎn)品: OE19 細胞, TE-10 細胞, TE-1 細胞, KYSE-510 細胞
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期刊:eLife
影響因子:7.7
引用產(chǎn)品: OE19 細胞, KYSE-510 細胞
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Q1:測OE-19細胞時發(fā)現(xiàn)細胞自帶少許綠色的熒光,如果后續(xù)要轉(zhuǎn)帶熒光的病毒,帶GFP的病毒這個有好的方法建議嗎
OE-19實驗室自測也有少許自帶綠色熒光,這個屬于細胞本身的熒光(目前具體原因還未知)。如果擔(dān)心自帶熒光和GFP干擾的話,可以考慮紅色的標(biāo)簽的抗體,比如mCherry抗體(紅色熒光染料)

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