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大鼠骨髓間充質(zhì)干細(xì)胞
Rat Bone Marrow Mesenchymal Stem Cells
貨號(hào)：CP-R131
規(guī)格：5×10?Cells/T25培養(yǎng)瓶
價(jià)格： ￥3380
產(chǎn)品概述
大鼠骨髓間充質(zhì)干細(xì)胞
Cat NO.: CP-R131
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產(chǎn)品名稱：大鼠骨髓間充質(zhì)干細(xì)胞
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英文簡(jiǎn)稱：BMSCs,MSCs
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組織來源：骨髓
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產(chǎn)品規(guī)格：5×105cells/T25細(xì)胞培養(yǎng)瓶
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細(xì)胞簡(jiǎn)介：
大鼠骨髓間充質(zhì)干細(xì)胞(BMSC)分離自骨髓；骨髓是機(jī)體的造血組織，位于身體的許多骨骼內(nèi)。成年動(dòng)物的骨髓分兩種：紅骨髓和黃骨髓。紅骨髓能制造紅細(xì)胞、血小板和各種白細(xì)胞。血小板有止血作用，白細(xì)胞能殺滅與抑制各種病原體，包括細(xì)菌、病毒等；某些淋巴細(xì)胞能制造抗體。因此，骨髓不但是造血器官，它還是重要的免疫器官。骨髓是存在于長(zhǎng)骨(如肱骨、股骨)的骨髓腔和扁平骨(如髂骨)的稀松骨質(zhì)間的網(wǎng)眼中，是一種海綿狀的組織，能產(chǎn)生血細(xì)胞的骨髓略呈紅色，稱為紅骨髓。出生時(shí)，紅骨髓充滿全身骨髓腔，隨著年齡增大，脂肪細(xì)胞增多，相當(dāng)部分紅骨髓被黃骨髓取代，最后幾乎只有扁平骨骨髓腔中有紅骨髓。骨髓基質(zhì)系統(tǒng)內(nèi)存在的骨髓間充質(zhì)干細(xì)胞是一種除造血干細(xì)胞以外的、具有高度自我更新能力和多向分化潛能的干細(xì)胞，可以向骨、軟骨、肌組織、皮膚、脂肪、神經(jīng)等多種組織分化，因此可以作為組織工程中的種子細(xì)胞。在骨髓中，BMSC占骨髓有核細(xì)胞總數(shù)的0.001%-0.1%，含量極低。骨髓間充質(zhì)干細(xì)胞的體外培養(yǎng)條件要求較高，在培養(yǎng)過程中，受貼壁時(shí)間、種植密度、血清含量、培養(yǎng)溫度和培養(yǎng)基pH值等條件的影響。
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方法簡(jiǎn)介：
普諾賽實(shí)驗(yàn)室分離的大鼠骨髓間充質(zhì)干細(xì)胞采用沖洗骨髓、密度梯度離心、差速貼壁法結(jié)合培養(yǎng)基篩選制備而來，細(xì)胞總量約為5×10?cells/瓶。
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質(zhì)量檢測(cè)：
普諾賽實(shí)驗(yàn)室分離的大鼠骨髓間充質(zhì)干細(xì)胞經(jīng)CD90或CD44免疫熒光鑒定，純度可達(dá)90%以上，且不含有HIV-1、HBV、HCV、支原體、細(xì)菌、酵母和真菌等。
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培養(yǎng)信息：
包被條件 培養(yǎng)基 含F(xiàn)BS、生長(zhǎng)添加劑、Penicillin、Streptomycin等 產(chǎn)品貨號(hào) CM-R131 換液頻率 每2-3天換液一次 生長(zhǎng)特性 貼壁 細(xì)胞形態(tài) 成纖維細(xì)胞樣 傳代特性 可傳5代左右；3代以內(nèi)狀態(tài)最佳 消化液 0.25%胰蛋白酶 培養(yǎng)條件 氣相：空氣，95%；CO2，5% 大鼠骨髓間充質(zhì)干細(xì)胞體外培養(yǎng)周期有限；建議使用普諾賽配套的專用生長(zhǎng)培養(yǎng)基及正確的操作方法來培養(yǎng)，以此保證該細(xì)胞的最佳培養(yǎng)狀態(tài)。
產(chǎn)品手冊(cè)
參考文獻(xiàn)
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