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產(chǎn)品概述
名稱 | MT-4 (人T細(xì)胞白血病細(xì)胞) (STR鑒定正確)(僅提供凍存管) |
種屬 | 人 |
生長特性 | 懸浮細(xì)胞 |
細(xì)胞形態(tài) | 淋巴細(xì)胞樣 |
凍存條件 | 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認(rèn)) |
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 3×10^5-5×10^5cells/mL |
推薦換液頻率 | 2-3次/周 |
注意事項(xiàng) | 該細(xì)胞為懸浮細(xì)胞,請(qǐng)注意離心收集細(xì)胞懸液;請(qǐng)勿直接倒掉細(xì)胞培養(yǎng)液。 |
背景描述 | Derived from a 50 year old Japanese male with generalised lymphadenopathy and heptosplenomegaly (Adult T-cell Leukaemia) by co-culture of his peripheral leukocytes with male umbilical cord lymphocytes. The cells carry HTLV-1 and support the growth of HIV. The cells should be handled under laboratory containment level 3 conditions. |
組織來源 | 淋巴細(xì)胞 |
細(xì)胞類型 | 腫瘤細(xì)胞 |
腫瘤類型 | 淋巴瘤細(xì)胞 |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X,Y CSF1PO 11,12 D2S1338 18,23 D3S1358 17 D5S818 10,11 D7S820 8,10 D8S1179 10,15 D13S317 12 D16S539 9,12 D18S51 13 D19S433 14.2,15.2 D21S11 28,30.3 FGA 23 PentaD 10,13 PentaE 5,15 TH01 7 TPOX 11 vWA 17,18 D6S1043 12 D12S391 18,21 D2S441 10 -
STR鑒定圖
參考文獻(xiàn)
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An acquired phosphatidylinositol 4-phosphate transport initiates T-cell deterioration and leukemogenesis(2022/07/29)
作者:Wenbin Zhong, Weize Lin, Yingjie Yang
期刊:Nature Communications
DOI:10.1038/s41467-022-32104-7
影響因子 :16.600
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