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0.25%胰蛋白酶溶液(含EDTA，溶于PBS)
貨號(hào)：PB180225
規(guī)格：125mL/500mL
價(jià)格： ￥68/￥228
產(chǎn)品概述
胰蛋白酶（Trypsin）是一種絲氨酸水解酶，它能把多肽鏈中賴氨酸和精氨酸殘基中的羧基側(cè)切段，水解細(xì)胞間的蛋白質(zhì)，破壞細(xì)胞間的連接，從而使組織或貼壁細(xì)胞離散成單個(gè)細(xì)胞。胰酶分散細(xì)胞的活性與組織或細(xì)胞的特性、胰酶濃度、溫度和作用時(shí)間有關(guān)，在PH 8.0和37℃時(shí)，胰酶的作用能力最強(qiáng)，因此使用胰酶時(shí)，應(yīng)把握好濃度、溫度和時(shí)間，以免消化過(guò)度造成細(xì)胞損傷。
一般常用胰酶的工作濃度為0.25%，而半貼壁細(xì)胞或?qū)σ让该舾械募?xì)胞常采用低濃度（0.05%）的胰酶進(jìn)行細(xì)胞消化。由于EDTA能夠螯合Ca2+和Mg2+，從而破壞細(xì)胞連接促進(jìn)細(xì)胞的解離，因此在胰酶溶液中常常會(huì)加入一定量的EDTA混合使用，以增強(qiáng)解離效果。
產(chǎn)品信息
形態(tài) | 液體 |
規(guī)格 | 125mL/500mL |
溶劑 | 10mM PBS |
胰蛋白酶濃度 | 2.5g/L |
EDTA?2Na | 0.2g/L |
酚紅指示劑 | 無(wú) |
儲(chǔ)存條件 | -5~-20℃ |
運(yùn)輸條件 | 低溫 |
有效期 | 12個(gè)月 |
注意事項(xiàng)
1、由于不同的組織或者細(xì)胞對(duì)胰酶的作用反應(yīng)不一樣，因此操作人員應(yīng)根據(jù)實(shí)際情況，確定最佳消化時(shí)間；消化細(xì)胞時(shí)間不宜過(guò)長(zhǎng)，否則會(huì)影響細(xì)胞貼壁和生長(zhǎng)狀況；
2、使用本產(chǎn)品時(shí)應(yīng)注意無(wú)菌操作，避免污染；
3、不宜長(zhǎng)時(shí)間放置于室溫環(huán)境或4℃長(zhǎng)期保存；
4、2-8℃中解凍，搖勻后使用，切忌反復(fù)凍融，用量較少時(shí)建議分裝凍存。
5、本產(chǎn)品僅用于科研或進(jìn)一步研究使用，不用于診斷和治療。
產(chǎn)品手冊(cè)
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