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產(chǎn)品概述
| 名稱 | EA.hy926 (人臍靜脈細(xì)胞融合細(xì)胞) (STR鑒定正確) |
| 別稱 | EA. hy 926; EA hy 926; EAhy 926; EAHY-926; EA.Hy926; EA.hy926; EAhy926; EaHy926; Eahy926 |
| 種屬 | 人 |
| 生長(zhǎng)特性 | 貼壁細(xì)胞 |
| 細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認(rèn)） |
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
|
| 推薦傳代比例 | 1:3-1:4 |
| 推薦換液頻率 | 2-3次/周 |
| 背景描述 | EA.hy926細(xì)胞是在PEG脅迫下，將原代培養(yǎng)的人臍靜脈細(xì)胞與抗硫鳥嘌呤的A549克隆株融合，構(gòu)建的一株人臍靜脈細(xì)胞株。融合子在HAT培養(yǎng)基上生長(zhǎng)，并以Ⅷ因子相關(guān)抗原篩選，EA.hy926細(xì)胞已經(jīng)過(guò)100次以上的群體倍增(PDLs)。電鏡照片顯示，Weibel-Palade小體的細(xì)胞質(zhì)分布以及組織特異性的細(xì)胞器，分化的內(nèi)皮細(xì)胞特性如血管生成，體內(nèi)平衡-血栓形成，血壓及炎癥反應(yīng)。 |
| 組織來(lái)源 | 臍靜脈/體細(xì)胞雜交 |
| 細(xì)胞類型 | 雜交瘤細(xì)胞 |
| 生物安全等級(jí) | BSL-1 |
| 倍增時(shí)間 | ~12-24 hours |
| 抗原表達(dá)情況 | Factor VIII-related antigen; Homo sapiens, expressed |
| 基因表達(dá)情況 | Factor VIII-related antigen; Homo sapiens. |
| 保藏機(jī)構(gòu) | ATCC; CRL-2922 |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X CSF1PO 10,11,12 D2S1338 22,24 D3S1358 15,16 D5S818 11 D7S820 8,9,10 D8S1179 13 D13S317 11 D16S539 11,12 D18S51 13,15,17 D19S433 13,14 D21S11 28,29,32 FGA 22,23 PentaD 9,11 PentaE 7,11,12 TH01 6,8,9.3 TPOX 8,9 vWA 14,17 D6S1043 11,12,22 D12S391 15,18 D2S441 10,11,14 -
STR鑒定圖
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參考文獻(xiàn)
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Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation(2023/10/13)
作者：Jiayu Liu, Chuanrong Zhao, Xue Xiao
期刊：Nature Communications
DOI：10.1038/s41467-023-42341-z
影響因子 ：16.600
引用產(chǎn)品：CL-0272
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Core–Shell Filament with Excellent Wound Healing Property Made of Cellulose Nanofibrils and Guar Gum via Interfacial Polyelectrolyte Complexation Spinning(2022/11/26)
作者：Meiyan Wu, Yinuo Liu, Chao Liu, Qiu Cui, Xin Zheng, Pedram Fatehi, Bin Li
期刊：Small
影響因子 ：13.300
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CD44 targeting nanodrug based on chondroitin sulfate for melanoma therapy by inducing mitochondrial apoptosis pathways(2023/08/02)
作者：Yan Li, Huiwen Hou, Zengmei Liu
期刊：CARBOHYDRATE POLYMERS
DOI：10.1016/j.carbpol.2023.121255
影響因子 ：11.200
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Chimeric Peptides Quickly Modify the Surface of Personalized 3D Printing Titanium Implants to Promote Osseointegration(2021/07/14)
作者：Zhezhe Zhao, Shiqing Ma, Chenxuan Wu
期刊：ACS Applied Materials & Interfaces
影響因子 ：9.500
引用產(chǎn)品：CL-0272
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A dual Keap1 and p47phox inhibitor Ginsenoside Rb1 ameliorates high glucose/ox-LDL-induced endothelial cell injury and atherosclerosis(2022/09/26)
作者：Zichao Wang, Kaiming Niu, Yujie Wu
期刊：Cell Death & Disease
DOI：10.1038/s41419-022-05274-x
影響因子 ：9.000
引用產(chǎn)品：CL-0272
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Chondroitin sulfate-modified antiangiogenic peptide conjugate induces cell apoptosis via the mitochondria-mediated pathway to perform antitumor activity(2024/02/29)
作者：Yan Li, Jiaai Fu, Huiwen Hou
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2024.129671
影響因子 ：8.200
引用產(chǎn)品：CL-0272
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Carboxy-terminal telopeptide levels of type I collagen hydrogels modulated the encapsulated cell fate for regenerative medicine(2022/12/22)
作者：Chuan Niu, Ying Xiong, Liping Yang
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2022.12.186
影響因子 ：8.200
引用產(chǎn)品：CL-0272
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Dynamic RGD ligands derived from highly mobile cyclodextrins regulate spreading and proliferation of endothelial cells to promote vasculogenesis(2024/04/16)
作者：Xuanshu Zhong, Shulei Zhang, Han Wang
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2024.131667
影響因子 ：8.200
引用產(chǎn)品：CL-0272
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Fabrication and performance evaluation of PLCL-hCOLIII small-diameter vascular grafts crosslinked with procyanidins(2023/08/15)
作者：Han Wang, Yonghao Xiao, Zhiping Fang
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2023.126293
影響因子 ：8.200
引用產(chǎn)品：CL-0272
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Enhancing cellular behavior in repaired tissue via silk fibroin-integrated triboelectric nanogenerators(2024/05/24)
作者：Zhelin Li, Shuxing Xu, Zijie Xu
期刊：Microsystems & Nanoengineering
DOI：10.1038/s41378-024-00694-5
影響因子 ：7.900
引用產(chǎn)品：CL-0272
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Biomimetic nanoplatform with selectively positioned indocyanine green for accurate sentinel lymph node imaging(2023/11/16)
作者：Wenjing Cheng, Xiangbai Wu, Shi Yu
期刊：Nanoscale
影響因子 ：6.700
引用產(chǎn)品：CL-0272
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Osteo-angiogenic and antibacterial activity of a multifunctional micro-porous coating on zirconium alloy(2022/08/06)
作者：Xiaoning Xu, Xiaohui Sun, Xinrong Tan
期刊：APPLIED SURFACE SCIENCE
DOI：10.1016/j.apsusc.2022.154465
影響因子 ：6.700
引用產(chǎn)品：CL-0272
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Osteo-angiogenic activity of a micro/nano hierarchical SrSi-codoped hydroxyapatite coating on zirconium alloy(2024/05/13)
作者：Xiaoning Xu, Kaige Wang, Xinrong Tan
期刊：Journal of Materials Research and Technology-JMR&T
DOI：10.1016/j.jmrt.2024.05.067
影響因子 ：6.400
引用產(chǎn)品：CL-0272
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Salvianolic Acid Ameliorates Pressure Overload-Induced Cardiac Endothelial Dysfunction via Activating HIF1α/HSF1/CD31 Pathway(2022/09/17)
作者：Na Li, Weijian Hang, Hongyang Shu
期刊：AMERICAN JOURNAL OF CHINESE MEDICINE
影響因子 ：6.005
引用產(chǎn)品：CL-0272
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PLA-HPG based coating enhanced anti-biofilm and wound healing of Shikonin in MRSA-infected burn wound(2023/08/10)
作者：Huiyu Han, Lianheng Chen, Shu Liang
期刊：Frontiers in Bioengineering and Biotechnology
DOI：10.3389/fbioe.2023.1243525
影響因子 ：5.700
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Porphyromonas gingivalis OMVs promoting endothelial dysfunction via the STING pathway in periodontitis(2024/05/02)
作者：Zhenwei Chen, Ye Cao, Wenxiu Jiang
期刊：ORAL DISEASES
影響因子 ：3.800
引用產(chǎn)品：CL-0272
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Platelet-rich plasma promotes diabetic ulcer repair through inhibition of ferroptosis(2022/10/01)
作者：Li Chen, Daoai Wu, Lili Zhou
期刊：Annals of Translational Medicine
影響因子 ：3.616
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Study on the effect of type III recombinant humanized collagen on human vascular endothelial cells(2023/11/29)
作者：Dr. Han Wang, Dr. Yonghao Xiao, Dr. Yuanguo Zhang
期刊：Tissue Engineering Part C-Methods
影響因子 ：3.000
引用產(chǎn)品：CL-0272
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Effect of High Magnesium and Astragaloside IV on Vascular Endothelial Cells(2024/05/09)
作者：Hongfei Qi, Teng Ge, Kun Wang
期刊：CELL BIOCHEMISTRY AND BIOPHYSICS
DOI：10.1007/s12013-024-01250-8
影響因子 ：2.600
引用產(chǎn)品：CL-0272
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Human umbilical cord mesenchymal stem cells overexpressing RUNX1 promote tendon-bone healing by inhibiting osteolysis, enhancing osteogenesis and promoting angiogenesis(2024/01/05)
作者：Dan Guo, Jian Yang, Dianwei Liu
期刊：Genes & Genomics
DOI：10.1007/s13258-023-01478-3
影響因子 ：2.100
引用產(chǎn)品：CL-0272
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產(chǎn)品資料
識(shí)別碼示意圖
![SW579 [SW 579; SW-579] (人甲狀腺鱗癌細(xì)胞) (STR鑒定正確)](/Public/upfile/pic/xbx/CL-0224-1.jpg)
娜胡文君.png "邱國(guó)娜/胡文君")
.png "梅文強(qiáng)")
