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產(chǎn)品概述
| 名稱 | B16 (小鼠黑色素瘤細(xì)胞) (STR鑒定正確) |
| 別稱 | B-16; B16 melanoma; B16 subline B78; B78 |
| 種屬 | C57BL/6小鼠 |
| 生長特性 | 貼壁細(xì)胞 |
| 細(xì)胞形態(tài) | 成纖維細(xì)胞樣 |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認(rèn)） |
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
|
| 推薦傳代比例 | 1:3-1:4 |
| 推薦換液頻率 | 2-3次/周 |
| 注意事項 | B16系列細(xì)胞，使用DMEM培養(yǎng)時可能出現(xiàn)黑色素快速積累，細(xì)胞不增殖或死亡的情況，該細(xì)胞正常擴增凍存請使用RPMI-1640完培，若您需要進行分泌黑色素等后續(xù)相關(guān)實驗，可以在擴增凍存細(xì)胞后更換為DMEM培養(yǎng)來滿足實驗設(shè)計。 |
| 背景描述 | B16細(xì)胞是一株小鼠黑素瘤細(xì)胞。 |
| 年齡（性別） | 不詳 |
| 組織來源 | 黑素瘤 |
| 細(xì)胞類型 | 腫瘤細(xì)胞 |
| 腫瘤類型 | 黑色素瘤細(xì)胞 |
| 生物安全等級 | BSL-1 |
| 保藏機構(gòu) | JCRB; JCRB0202 KCB; KCB 93030YJ RCB; RCB1283 TKG; TKG 0144 |
STR鑒定
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STR位點信息
18-3 15,16 6-7 15,16 5-5 16,20 X-1 28 1-2 19,20 7-1 26.2 8-1 16,17 1-1 17,18 19-2 13 15-3 22.3,23.3 12-1 17,18 6-4 18,19 4-2 20.3,21.3 3-2 14,15 2-1 16 13-1 17.1,18.1
11-2 16,17 17-2 15,16,17 -
STR鑒定圖
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參考文獻
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In Situ Self-Assembly Nanomicelle Microneedles for Enhanced Photoimmunotherapy via Autophagy Regulation Strategy(2021/02/23)
作者：Minglong Chen, Dan Yang, Ying Sun
期刊：ACS Nano
影響因子 ：17.100
引用產(chǎn)品：CL-0029
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Impact of particle size and pH on protein corona formation of solid lipid nanoparticles: A proof-of-concept study(2020/10/29)
作者：Wenhao Wang, Zhengwei Huang, Yanbei Li
期刊：Acta Pharmaceutica Sinica B
DOI：10.1016/j.apsb.2020.10.023
影響因子 ：14.500
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CD44 targeting nanodrug based on chondroitin sulfate for melanoma therapy by inducing mitochondrial apoptosis pathways(2023/08/02)
作者：Yan Li, Huiwen Hou, Zengmei Liu
期刊：CARBOHYDRATE POLYMERS
DOI：10.1016/j.carbpol.2023.121255
影響因子 ：11.200
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Microneedle-assisted vaccination combined with autophagy regulation for antitumor immunotherapy(2023/04/24)
作者：Dan Yang, Minglong Chen, Ying Sun
期刊：JOURNAL OF CONTROLLED RELEASE
DOI：10.1016/j.jconrel.2023.04.031
影響因子 ：10.800
引用產(chǎn)品：CL-0029
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ATP-adenosine axis regulation combined with microneedle assisted photoimmunotherapy to boost the immunotherapy efficiency(2024/01/23)
作者：Chaonan Shi, Minglong Chen, Xiaodie Li
期刊：JOURNAL OF CONTROLLED RELEASE
DOI：10.1016/j.jconrel.2024.01.035
影響因子 ：10.800
引用產(chǎn)品：CL-0029
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A novel multifunctional microneedle patch for synergistic photothermal- gas therapy against maxillofacial malignant melanoma and associated skin defects(2024/04/23)
作者：Shaojie Dong, Yuwei Zhang, Yifei Zhang
期刊：JOURNAL OF NANOBIOTECHNOLOGY
DOI：10.1186/s12951-024-02409-4
影響因子 ：10.200
引用產(chǎn)品：CL-0029
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Extracellular matrix modulating enzyme functionalized biomimetic Au nanoplatform-mediated enhanced tumor penetration and synergistic antitumor therapy for pancreatic cancer(2022/12/10)
作者：Xiaoyan Yang, Jinguo Zhang, Qiaomei Zhou
期刊：JOURNAL OF NANOBIOTECHNOLOGY
DOI：10.1186/s12951-022-01738-6
影響因子 ：10.200
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Delivery of mRNA Vaccine with 1, 2-Diesters-Derived Lipids Elicits Fast Liver Clearance for Safe and Effective Cancer Immunotherapy(2023/11/21)
作者：Yuandong Xu, Yuexiao Hu, Heng Xia
期刊：Advanced Healthcare Materials
影響因子 ：10.000
引用產(chǎn)品：CL-0029
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Synthesis of iron-boride/carbon-nitride composites and their applications in chemodynamic therapy(2023/12/14)
作者：Xiaoran Xu, Haixu Zhao, JiaJia Wang
期刊：JOURNAL OF COLLOID AND INTERFACE SCIENCE
DOI：10.1016/j.jcis.2023.12.078
影響因子 ：9.900
引用產(chǎn)品：CL-0029
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Janus-Inspired Core–Shell Structure Hydrogel Programmatically Releases Melatonin for Reconstruction of Postoperative Bone Tumor(2023/01/05)
作者：Wei Huang, Xiaoyue Wu, Yifan Zhao
期刊：ACS Applied Materials & Interfaces
影響因子 ：9.500
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High-contrast tumor imaging via a de novo designed aminopeptidase N targeted fluorogenic probe(2023/12/04)
作者：Xing Gao, Chengyu Fan, Huiling Wang
期刊：SENSORS AND ACTUATORS B-CHEMICAL
影響因子 ：8.400
引用產(chǎn)品：CL-0029
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In situ administration of temperature-sensitive hydrogel composite loading paclitaxel microspheres and cisplatin for the treatment of melanoma(2023/02/10)
作者：Yanlin Liu, Wenqiong Ma, Ping Zhou, Qian Wen, Qinglian Wen, Yun Lu, Ling Zhao, Huan Shi, Jie Dai, Jianmei Li, Shaozhi Fu
期刊：BIOMEDICINE & PHARMACOTHERAPY
DOI：10.1016/j.biopha.2023.114380
影響因子 ：7.500
引用產(chǎn)品：CL-0029
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Dissolving microneedles integrated with pH-responsive micelles containing AIEgen with ultra-photostability for enhancing melanoma photothermal therapy(2020/09/10)
作者：Sihui Wei, Guilan Quan, Chao Lu
期刊：Biomaterials Science
影響因子 ：6.600
引用產(chǎn)品：CL-0029
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Promotion of Melanoma Cell Proliferation by Cyclic Straining through Regulatory Morphogenesis(2022/10/06)
作者：Siyuan Huang, Zhu Chen, Xiaoqiang Hou
期刊：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影響因子 ：5.600
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A Digital Controlled Pulse Generator for a Possible Tumor Therapy Combining Irreversible Electroporation With Nanosecond Pulse Stimulation(2020/04/17)
作者：Xin Rao, Xiaodong Chen, Jun Zhou
期刊：IEEE Transactions on Biomedical Circuits and Systems
DOI：10.1109/TBCAS.2020.2987376
影響因子 ：5.100
引用產(chǎn)品：CL-0029
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A colorimetric and near -infrared ratiometric fluorescent probe for the determination of endogenous tyrosinase activity based on cyanine aggregation(2019/09/21)
作者：Peng Zhang, Shasha Li, Caixia Fu
期刊：Analyst
影響因子 ：4.200
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Peptide OA-VI12 restrains melanogenesis in B16 cells and C57B/6 mouse ear skin via the miR-122-5p/Mitf/Tyr axis(2023/10/04)
作者：Junsong Wang, Yilin Li, Chengan Feng
期刊：AMINO ACIDS
DOI：10.1007/s00726-023-03341-x
影響因子 ：3.500
引用產(chǎn)品：CL-0029
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Newly identified peptide Nigrocin-OA27 inhibits UVB induced melanin production via the MITF/TYR pathway(2024/04/11)
作者：Jiayi Li, Saige Yin, Ziqi Wei
期刊：PEPTIDES
DOI：10.1016/j.peptides.2024.171215
影響因子 ：3.000
引用產(chǎn)品：CL-0029
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CREB1 regulates KPNA2 by inhibiting mir-495-3p transcription to control melanoma progression(2022/12/15)
作者：Xuerui Geng, Xiujuan Qiu, Jun Gao
期刊：BMC Molecular and Cell Biology
DOI：10.1186/s12860-022-00446-1
影響因子 ：2.800
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