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![U-2 OS [U2OS] (人骨肉瘤細胞) (STR鑒定正確)](/Public/upfile/pic/xbx/CL-0236-1.jpg)
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U-2 OS [U2OS] (人骨肉瘤細胞) (STR鑒定正確)
貨號：CL-0236
規(guī)格：1×10?cells/T25培養(yǎng)瓶
價格： ￥1200
生長培養(yǎng)基：McCoy's 5A＋10% FBS＋1% P/S
產(chǎn)品概述
| 名稱 | U-2 OS [U2OS] (人骨肉瘤細胞) (STR鑒定正確) |
| 別稱 | U-2 OS; U-2OS; U-2-OS; U2-OS; U20-S; U20S; 2T |
| 種屬 | 人 |
| 生長特性 | 貼壁細胞 |
| 細胞形態(tài) | 上皮細胞樣 |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認） |
生長培養(yǎng)基:
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
|
| 推薦傳代比例 | 1:3-1:4 |
| 推薦換液頻率 | 2-3次/周 |
| 背景描述 | U-2 OS細胞(原名2T)是由Ponten·J和Saksela·E在1964年從一名15歲女孩的脛骨中等分化的肉瘤中分離建立的。U-2 OS細胞與WI-38細胞共培養(yǎng)或用抗猴空泡病毒SV40、呼吸道合胞病毒RSV或腺病毒的補體結(jié)合試驗(CF法)未檢測到病毒。U-2 OS細胞表達胰島素樣生長因子I、II(IGF-I、IGF-II)受體和骨肉瘤衍生生長因子(ODGF)。 |
| 年齡（性別） | 女性；15歲 |
| 組織來源 | 骨；骨肉瘤 |
| 細胞類型 | 腫瘤細胞 |
| 腫瘤類型 | 肉瘤細胞 |
| 生物安全等級 | BSL-1 |
| 倍增時間 | ~25-30 hours |
| 受體表達情況 | insulin-like growth factor I (IGF-I);insulin-like growth factor II (IGF II) |
| 抗原表達情況 | Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-) |
| 基因表達情況 | osteosacoma derived growth factor (ODGF), Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-) |
| 保藏機構(gòu) | ATCC; HTB-96 DSMZ; ACC-785 ECACC; 92022711 |
STR鑒定
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STR位點信息
Amelogenin X CSF1PO 13 D2S1338 20,24 D3S1358 16 D5S818 11 D7S820 11,12 D8S1179 12,14 D13S317 13 D16S539 11,12 D18S51 14 D19S433 15 D21S11 31 FGA 20,21 PentaD 9 PentaE 10,13 TH01 6,9.3 TPOX 11,12 vWA 14,18 D6S1043 11 D12S391 19,20 D2S441 10,14 -
STR鑒定圖
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參考文獻
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Intrinsic bioactivity of black phosphorus nanomaterials on mitotic centrosome destabilization through suppression of PLK1 kinase(2021/08/05)
作者：Ximing Shao, Zhihao Ding, Wenhua Zhou
期刊：Nature Nanotechnology
DOI：10.1038/s41565-021-00952-x
影響因子 ：38.300
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期刊：ADVANCED FUNCTIONAL MATERIALS
影響因子 ：19.000
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作者：Ying Zheng, Zhiwei Ye, Xue Zhang
期刊：Journal of the American Chemical Society
影響因子 ：15.000
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Engineered biomimetic nanoreactor for synergistic photodynamic-chemotherapy against hypoxic tumor(2022/09/22)
作者：Haoyu Guo, Lutong Wang, Wei Wu
期刊：JOURNAL OF CONTROLLED RELEASE
DOI：10.1016/j.jconrel.2022.09.020
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Bone-targeting exosome nanoparticles activate Keap1 / Nrf2 / GPX4 signaling pathway to induce ferroptosis in osteosarcoma cells(2023/09/30)
作者：Wenkai Chen, Zongguang Li, Naichun Yu
期刊：JOURNAL OF NANOBIOTECHNOLOGY
DOI：10.1186/s12951-023-02129-1
影響因子 ：10.200
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Janus-Inspired Core–Shell Structure Hydrogel Programmatically Releases Melatonin for Reconstruction of Postoperative Bone Tumor(2023/01/05)
作者：Wei Huang, Xiaoyue Wu, Yifan Zhao
期刊：ACS Applied Materials & Interfaces
影響因子 ：9.500
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Targeting visualization of malignant tumor based on the alteration of DWI signal generated by hTERT promoter–driven AQP1 overexpression(2022/01/19)
作者：Liang Zhang, Mingfu Gong, Sheng Lei
期刊：European Journal Of Nuclear Medicine And Molecular Imaging
DOI：10.1007/s00259-022-05684-1
影響因子 ：9.100
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lncRNA MELTF-AS1 facilitates osteosarcoma metastasis by modulating MMP14 expression(2021/08/26)
作者：Lei Ding, Taiyuan Liu, Yuan Qu
期刊：Molecular Therapy-Nucleic Acids
DOI：10.1016/j.omtn.2021.08.022
影響因子 ：8.800
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Comprehensive analysis of a glycolysis and cholesterol synthesis-related genes signature for predicting prognosis and immune landscape in osteosarcoma(2022/12/23)
作者：Fangxing Xu, Jinglong Yan, Zhibin Peng
期刊：Frontiers in Immunology
DOI：10.3389/fimmu.2022.1096009
影響因子 ：8.786
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期刊：CELLULAR & MOLECULAR BIOLOGY LETTERS
DOI：10.1186/s11658-024-00544-2
影響因子 ：8.300
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Subtle Structural Translation Magically Modulates the Super-Resolution Imaging of Self-Blinking Rhodamines(2023/02/14)
作者：Ying Zheng, Zhiwei Ye, Yi Xiao
期刊：Analytical Chemistry
DOI：10.1021/acs.analchem.2c05298
影響因子 ：7.400
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CKB affects human osteosarcoma progression by regulating the p53 pathway(2022/10/15)
作者：Fengxin Zhou, Xinli Dou, Chenguang Li
期刊：American Journal of Cancer Research
DOI：PMID:36381321
影響因子 ：5.942
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What role does GPR65 play in the progression of osteosarcoma? Its mechanism and clinical significance(2024/01/13)
作者：Jin Qi, Sihang Liu, Zhirui Zhang
期刊：Cancer Cell International
DOI：10.1186/s12935-024-03216-5
影響因子 ：5.800
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Roles of PTEN gene methylation in Se-CQDs induced mitochondrial apoptosis of osteosarcoma cells(2024/01/16)
作者：Huahua Su, Yan Lv, Lixian Zhu
期刊：COLLOIDS AND SURFACES B-BIOINTERFACES
DOI：10.1016/j.colsurfb.2024.113757
影響因子 ：5.800
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A1BG-AS1 promotes the biological functions of osteosarcoma cells via regulating the microRNA-148a-3p/USP22 axis and stabilizing the expression of SIRT1 through deubiquitinase function(2023/10/03)
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DOI：10.1080/14728222.2023.2263908
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A mitochondria-related signature for predicting immune microenvironment and therapeutic response in osteosarcoma(2022/12/01)
作者：Lina Zhang, Song Wu, Junjie Huang
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2-Hydroxy-3-methylanthraquinone inhibits homologous recombination repair in osteosarcoma through the MYC-CHK1-RAD51 axis(2023/01/30)
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DOI：10.1186/s10020-023-00611-y
影響因子 ：5.700
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Solasonine Inhibits Cancer Stemness and Metastasis by Modulating Glucose Metabolism via Wnt/β-Catenin/Snail Pathway in Osteosarcoma(2023/06/28)
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DOI：10.1186/s10020-024-00778-y
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MicroRNA PC-3p-2869 Regulates Antler Growth and Inhibits Proliferation and Migration of Human Osteosarcoma and Chondrosarcoma Cells by Targeting CDK8, EEF1A1, and NTN1(2023/06/29)
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m7G-related genes predict prognosis and affect the immune microenvironment and drug sensitivity in osteosarcoma(2023/08/16)
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DOI：10.3389/fphar.2023.1158775
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Targeted PLK1 suppression through RNA interference mediated by high-fidelity Cas13d mitigates osteosarcoma progression via TGF-β/Smad3 signalling(2024/05/23)
作者：Yi Yuan, Daigui Cao, Anwei Zhang
期刊：JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
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Circular RNA hsa_circ_001350 contributes to osteosarcoma progression by regulating microRNA-578/CCR4-NOT transcription complex and subunit 7/Wnt signaling(2023/06/15)
作者：Rujin Xu, Xingyuan Ci, Fang He
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影響因子 ：5.300
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Trifluoperazine activates AMPK / mTOR / ULK1 signaling pathway to induce mitophagy in osteosarcoma cells(2024/02/13)
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影響因子 ：5.100
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Zinc finger Asp-His-His-Cys palmitoyl -acyltransferase 19 accelerates tumor progression through wnt/β-catenin pathway and is upregulated by miR-940 in osteosarcoma(2022/03/17)
作者：Shuhong Liang, Xiaojian Zhang, Jun Li
期刊：Bioengineered
DOI：10.1080/21655979.2022.2040827
影響因子 ：4.900
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Sulfatinib, a novel multi-targeted tyrosine kinase inhibitor of FGFR1, CSF1R, and VEGFR1–3, suppresses osteosarcoma proliferation and invasion via dual role in tumor cells and tumor microenvironment(2023/06/08)
作者：Song Liao, Jianxiong Li, Song Gao
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影響因子 ：4.700
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Zyxin Inhibits the Proliferation, Migration, and Invasion of Osteosarcoma via Rap1-Mediated Inhibition of the MEK/ERK Signaling Pathway(2023/08/21)
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A ferroptosis-related gene signature associated with immune landscape and therapeutic response in osteosarcoma.(2022/11/11)
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作者：Xu Cai, Shaihong Zhu
期刊：Genomics
DOI：10.1016/j.ygeno.2023.110595
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Develop a Novel Signature to Predict the Survival and Affect the Immune Microenvironment of Osteosarcoma Patients: Anoikis-Related Genes(2024/03/27)
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期刊：Journal of Immunology Research
影響因子 ：4.100
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Osteoblast-derived exosomes promote osteogenic differentiation of osteosarcoma cells via URG4/Wnt signaling pathway(2023/10/11)
作者：Yuanxi Leng, Jingtang Li, Zhisheng Long
期刊：BONE
DOI：10.1016/j.bone.2023.116933
影響因子 ：4.100
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Identification of an EMT-related gene-based prognostic signature in osteosarcoma(2023/04/27)
作者：Haoli Gong, Ye Tao, Sheng Xiao
期刊：Cancer Medicine
影響因子 ：4.000
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Down-regulation of PRR11 affects the proliferation, migration and invasion of osteosarcoma by inhibiting the Wnt/β-catenin pathway(2021/09/13)
作者：Ke Li,?Hongtao Yu,?Chunbing Zhao
期刊：Journal of Cancer
影響因子 ：3.900
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A short peptide LINC00665_18aa encoded by lncRNA LINC00665 suppresses the proliferation and migration of osteosarcoma cells through the regulation of the CREB1/RPS6KA3 interaction(2023/06/07)
作者：Junwei Pan, Ming Liu, Xiaozong Duan
期刊：PLoS One
DOI：10.1371/journal.pone.0286422
影響因子 ：3.700
引用產(chǎn)品：164210 164210-50 CL-0236 CL-0492 PB180120 PM150210
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Schisandrin B Inhibits Cell Viability and Migration, and Induces Cell Apoptosis by circ_0009112/miR-708-5p Axis Through PI3K/AKT Pathway in Osteosarcoma(2021/12/22)
作者：Bing Wang, Xiaowei Wang, Xing Tong
期刊：Frontiers in Genetics
影響因子 ：3.700
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Disulfidptosis-related PABPC3 promotes tumor progression and inhibits immune activity in osteosarcoma(2023/12/07)
作者：Yangbo Cao, Song Wu, Yishan Gu
期刊：JOURNAL OF GENE MEDICINE
DOI：10.1002/jgm.3641
影響因子 ：3.500
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Diverse cell death patterns-related signature for predicting prognosis and drug sensitivity of osteosarcoma patients(2023/10/20)
作者：Yanbin Shi, Song Wu, Xiaolin Zhang
期刊：JOURNAL OF GENE MEDICINE
DOI：10.1002/jgm.3613
影響因子 ：3.500
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Hsa_circ_0000591 drives osteosarcoma glycolysis and progression by sequestering miR-194-5p and elevating HK2 expression(2023/02/21)
作者：Dawei Wang, Yang Wang, Huadong Wang
期刊：CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
影響因子 ：2.900
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LPS combined with CD47mAb enhances the anti?osteosarcoma ability of macrophages(2023/03/28)
作者：Peng Yuan, Yukang Que, Yulei Liu
期刊：Oncology Letters
影響因子 ：2.900
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PROSER2 is a poor prognostic biomarker for patients with osteosarcoma and promotes proliferation, migration and invasion of osteosarcoma cells(2022/11/08)
作者：Zhengjiang Li, Yan Zhang, Yongkui Li, Shuxing Xing, Shunqiang Li, Jing Lyu, Zhaonan Ban
期刊：Experimental and Therapeutic Medicine
影響因子 ：2.700
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DEPDC1 and KIF4A synergistically inhibit the malignant biological behavior of osteosarcoma cells through Hippo signaling pathway(2023/02/27)
作者：Mingming Yang, Hang Zhang, Shichang Gao
期刊：Journal of Orthopaedic Surgery and Research
DOI：10.1186/s13018-023-03572-4
影響因子 ：2.600
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Exosomal miR-331-3p derived from chemoresistant osteosarcoma cells induces chemoresistance through autophagy(2023/11/22)
作者：Chenyang Meng, Yun Yang, Wei Feng
期刊：Journal of Orthopaedic Surgery and Research
DOI：10.1186/s13018-023-04338-8
影響因子 ：2.600
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-
Piperine improves the sensitivity of osteosarcoma cells to doxorubicin by inducing apoptosis and inhibiting the PI3K/AKT/GSK-3β pathway(2023/03/09)
作者：Yubin Qi, Lin Yao, Jianke Liu
期刊：Journal of Orthopaedic Surgery and Research
DOI：10.1186/s13018-023-03642-7
影響因子 ：2.600
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-
Circ_0003732 promotes osteosarcoma progression through regulating miR-377-3p/CPEB1 axis and Wnt/β-catenin signaling pathway(2022/01/01)
作者：Zheng Zhou, Tang Liu, Zhihong Li
期刊：Anti-Cancer Drugs
DOI：10.1097/CAD.0000000000001206
影響因子 ：2.389
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circATP2A2 promotes osteosarcoma progression by upregulating MYH9(2021/11/22)
作者：Xin Cao, Xianfeng Meng, Peng Fu
期刊：Open Medicine
影響因子 ：2.100
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Circular RNA circ_0096041 promotes osteosarcoma cell proliferation and migration via sponging miR-556-5p and regulating LIN28A expression(2024/02/29)
作者：Fulin Zhou, Hao Wu, Zhen Yin
期刊：CELLULAR AND MOLECULAR BIOLOGY
影響因子 ：1.600
引用產(chǎn)品：CL-0236
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