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MNNG/HOS Cl #5 [R-1059-D] (人骨肉瘤細(xì)胞) (STR鑒定正確)
貨號(hào):CL-0492
規(guī)格:1×10?cells/T25培養(yǎng)瓶
價(jià)格: ¥1600
生長(zhǎng)培養(yǎng)基:MEM(含NEAA)+10% FBS+1% P/S
產(chǎn)品概述
名稱 | MNNG/HOS Cl #5 [R-1059-D] (人骨肉瘤細(xì)胞) (STR鑒定正確) |
別稱 | MNNG/HOS; MNNG-HOS; HOS-MNNG; MNNGHOS; MNNG-HOS (Cl#5) ; MNNG/HOS Clone F-5; MNNG; R-1059-D; TE85; TE-85; HOS-TE85; Hos TE-85; HOS TE 85; HOS TE85; HOS (TE85) ; HOS (TE85) ; HOS (TE85, Clone F5) ; MNNG-HOS (TE 85, clone F-5) ; HOS-Te85; TE 85.T; TE 85 ClF-5; TE-85 clone 5 |
種屬 | 人 |
生長(zhǎng)特性 | 貼壁細(xì)胞 |
細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
凍存條件 | 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認(rèn)) |
生長(zhǎng)培養(yǎng)基:
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 1:2-1:4 |
推薦換液頻率 | 2-3次/周 |
背景描述 | MNNG/HOS Cl #5 [R-1059-D]細(xì)胞是HOS經(jīng)0.01mcg/ml MNNG(一種致癌的亞硝胺)轉(zhuǎn)化而來(lái)。MNNG/HOS Cl #5 [R-1059-D]細(xì)胞飽和濃度高,在軟瓊脂中的克隆形成率高,并能在裸鼠中成瘤。 |
年齡(性別) | 女性;13歲 |
組織來(lái)源 | 骨;骨肉瘤 |
細(xì)胞類型 | 腫瘤細(xì)胞 |
腫瘤類型 | 肉瘤細(xì)胞 |
生物安全等級(jí) | BSL-1 |
保藏機(jī)構(gòu) | ATCC; CRL-1547 ECACC; 87070201 |
STR鑒定
-
STR位點(diǎn)信息
Amelogenin X CSF1PO 12 D2S1338 24,25 D3S1358 15 D5S818 13 D7S820 11,12 D8S1179 11,14 D13S317 12 D16S539 10,13 D18S51 14 D19S433 13 D21S11 31.2 FGA 24 PentaD 9,10 PentaE 7,12 TH01 6 TPOX 8,11 vWA 18 D6S1043 18 D12S391 20 D2S441 10 -
STR鑒定圖
參考文獻(xiàn)
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