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人骨髓間充質(zhì)干細(xì)胞
Human Bone Marrow Mesenchymal Stem Cells
貨號：CP-H166
規(guī)格：5×10?Cells/T25培養(yǎng)瓶
價格： ￥7380
產(chǎn)品概述
人骨髓間充質(zhì)干細(xì)胞
Cat NO.: CP-H166
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產(chǎn)品名稱：人骨髓間充質(zhì)干細(xì)胞
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英文簡稱：BMSCs,MSCs
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組織來源：骨髓
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產(chǎn)品規(guī)格：5×105cells/T25細(xì)胞培養(yǎng)瓶
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細(xì)胞簡介：
人骨髓間充質(zhì)干細(xì)胞(BMSC)分離自骨髓；骨髓是機體的造血組織，位于身體的許多骨骼內(nèi)。成年動物的骨髓分兩種：紅骨髓和黃骨髓。紅骨髓能制造紅細(xì)胞、血小板和各種白細(xì)胞。血小板有止血作用，白細(xì)胞能殺滅與抑制各種病原體，包括細(xì)菌、病毒等；某些淋巴細(xì)胞能制造抗體。因此，骨髓不但是造血器官，它還是重要的免疫器官。骨髓是存在于長骨(如肱骨、股骨)的骨髓腔和扁平骨(如髂骨)的稀松骨質(zhì)間的網(wǎng)眼中，是一種海綿狀的組織，能產(chǎn)生血細(xì)胞的骨髓略呈紅色，稱為紅骨髓。出生時，紅骨髓充滿全身骨髓腔，隨著年齡增大，脂肪細(xì)胞增多，相當(dāng)部分紅骨髓被黃骨髓取代，最后幾乎只有扁平骨骨髓腔中有紅骨髓。骨髓基質(zhì)系統(tǒng)內(nèi)存在的骨髓間充質(zhì)干細(xì)胞是一種除造血干細(xì)胞以外的、具有高度自我更新能力和多向分化潛能的干細(xì)胞，可以向骨、軟骨、肌組織、皮膚、脂肪、神經(jīng)等多種組織分化，因此可以作為組織工程中的種子細(xì)胞。在骨髓中，BMSC占骨髓有核細(xì)胞總數(shù)的0.001%-0.1%，含量極低。骨髓間充質(zhì)干細(xì)胞的體外培養(yǎng)條件要求較高，在培養(yǎng)過程中，受貼壁時間、種植密度、血清含量、培養(yǎng)溫度和培養(yǎng)基pH值等條件的影響。
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方法簡介：
普諾賽實驗室分離的人骨髓間充質(zhì)干細(xì)胞采用沖洗骨髓、密度梯度離心、差速貼壁法結(jié)合培養(yǎng)基篩選制備而來，細(xì)胞總量約為5×10?cells/瓶。
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質(zhì)量檢測：
普諾賽實驗室分離的人骨髓間充質(zhì)干細(xì)胞經(jīng)CD29或CD90免疫熒光鑒定，純度可達90%以上，且不含有HIV-1、HBV、HCV、支原體、細(xì)菌、酵母和真菌等。
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培養(yǎng)信息：
包被條件 培養(yǎng)基 含F(xiàn)BS、生長添加劑、Penicillin、Streptomycin等 產(chǎn)品貨號 CM-H166 換液頻率 每2-3天換液一次 生長特性 貼壁 細(xì)胞形態(tài) 成纖維細(xì)胞樣 傳代特性 可傳3-5代左右；3代以內(nèi)狀態(tài)最佳 消化液 0.25%胰蛋白酶 培養(yǎng)條件 氣相：空氣，95%；CO2，5% 人骨髓間充質(zhì)干細(xì)胞體外培養(yǎng)周期有限；建議使用普諾賽配套的專用生長培養(yǎng)基及正確的操作方法來培養(yǎng)，以此保證該細(xì)胞的最佳培養(yǎng)狀態(tài)。
產(chǎn)品手冊
參考文獻
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Spontaneous immunomodulation and regulation of angiogenesis and osteogenesis by Sr/Cu-borosilicate glass (BSG) bone cement to repair critical bone defects(2022/11/09)
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Cell membrane vesicles derived from hBMSCs and hUVECs enhance bone regeneration(2024/04/09)
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Bioinformatic analysis of related immune cell infiltration and key genes in the progression of osteonecrosis of the femoral head(2024/01/11)
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作者：Liu Ansong, Chen Yong, Zhong Da, Wang Chenggong, Yu Mi, Liu Chao, Yang Zhijun, Chen Wenkang, Yin Ke
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YTHDF2-Mediated m6A methylation inhibition by miR27a as a protective mechanism against hormonal osteonecrosis in BMSCs(2024/05/06)
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期刊：BMC MUSCULOSKELETAL DISORDERS
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影響因子 ：2.300
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期刊：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
DOI：10.1007/s11626-023-00811-0
影響因子 ：2.100
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LncRNA TUG regulates osteogenic differentiation of bone marrow mesenchymal stem cells via miRNA-204/SIRT 1(2022/09/01)
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期刊：JOURNAL OF MUSCULOSKELETAL & NEURONAL INTERACTIONS
DOI：PMID:36046997
影響因子 ：1.900
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MicroRNA-4461 derived from bone marrow mesenchymal stem cell exosomes inhibits tumorigenesis by downregulating COPB2 expression in colorectal cancer(2019/10/21)
作者：Huili Chen, Jiujiang Li, Fei Jiang
期刊：Bioscience Biotechnology And Biochemistry
DOI：10.1080/09168451.2019.1677452
影響因子 ：1.600
引用產(chǎn)品：CP-H166
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