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產(chǎn)品概述
名稱 | NCTC 1469 (小鼠正常肝細(xì)胞) (STR鑒定正確) |
別稱 | NCTC clone 1469; NCTC1469 |
種屬 | 小鼠 |
生長(zhǎng)特性 | 半貼半懸 |
細(xì)胞形態(tài) | 圓形、上皮細(xì)胞樣 |
凍存條件 | 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認(rèn)) |
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 1:2-1:4 |
推薦換液頻率 | 2-3次/周 |
注意事項(xiàng) | 該細(xì)胞生長(zhǎng)狀態(tài)為半貼半懸,貼壁部分處理時(shí)需要用胰酶消化。培養(yǎng)時(shí),間隔處理時(shí)間過(guò)長(zhǎng)會(huì)導(dǎo)致細(xì)胞貼壁部分消化時(shí)間延長(zhǎng)。 |
背景描述 | NCTC 1469細(xì)胞是于1952年建系,源于正常C3H/An小鼠的肝臟組織;NCTC 1469細(xì)胞可表達(dá)H-2K抗原,鼠痘病毒陰性。 |
年齡(性別) | 新生 |
組織來(lái)源 | 肝臟 |
細(xì)胞類型 | 自發(fā)永生化細(xì)胞 |
生物安全等級(jí) | BSL-1 |
保藏機(jī)構(gòu) | ATCC; CCL-9.1 ECACC; 88111403 |
STR鑒定
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STR位點(diǎn)信息
18-3 16 6-7 12 5-5 15 X-1 26 1-2 16 7-1 26,27 8-1 14,16 1-1 10 19-2 11,12 15-3 25.3 12-1 16 6-4 18 4-2 20.3 3-2 14,15 2-1 9 13-1 17.1,18.1
11-2 16,18 17-2 15,16,17 -
STR鑒定圖
參考文獻(xiàn)
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Reprogramming Exosomes to Escape from Immune Surveillance for Mitochondrial Protection in Hepatic Ischemia-Reperfusion Injury(2024/01/01)
作者:Shanshan Liu, Xinyu Xiao, La Zhang
期刊:Theranostics
影響因子 :12.400
引用產(chǎn)品:CL-0407
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Inhibition of GCN2 alleviates hepatic steatosis and oxidative stress in obese mice: Involvement of NRF2 regulation(2021/12/22)
作者:Juntao Yuan, Zhuoran Yu, Junling Gao
期刊:Redox Biology
DOI:10.1016/j.redox.2021.102224
影響因子 :11.400
引用產(chǎn)品:CL-0407
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Natural long-chain saturated fatty acids doped LNPs enabling spleen selective mRNA translation and potent cancer immunotherapy(2023/09/08)
作者:Fazhan Wang, Meng Zhang, Meiling Tian
期刊:Nano Research
影響因子 :9.900
引用產(chǎn)品:CL-0407
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Resmetirom Ameliorates NASH-Model Mice by Suppressing STAT3 and NF-κB Signaling Pathways in an RGS5-Dependent Manner(2023/03/19)
作者:Xiaojing Wang, Liangjing Wang, Lin Geng
期刊:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
影響因子 :5.600
引用產(chǎn)品:CL-0407
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Periplaneta americana extract alleviates steatohepatitis in a mouse model by modulating HMGB1-mediated inflammatory response(2022/10/03)
作者:Yang Xiao, Chongqing Gao, Junru Wu
期刊:Frontiers in Pharmacology
影響因子 :5.600
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Dietary intake of fructose increases purine de novo synthesis: A crucial mechanism for hyperuricemia(2022/12/19)
作者:Pengfei Zhang, Huimin Sun, Xinyu Cheng
期刊:Frontiers in Nutrition
影響因子 :5.000
引用產(chǎn)品:CL-0407
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The Different Mechanisms of Lipid Accumulation in Hepatocytes Induced by Oleic Acid/Palmitic Acid and High-Fat Diet(2023/09/20)
作者:Miao Zhang, Xue Bai, Qian Du
期刊:MOLECULES
影響因子 :4.600
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Uncarboxylated Osteocalcin Decreases SCD1 by Activating AMPK to Alleviate Hepatocyte Lipid Accumulation(2023/03/31)
作者:Danqing Wang, Miao Zhang, Jiaojiao Xu
期刊:MOLECULES
影響因子 :4.600
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CBX7 silencing promoted liver regeneration by interacting with BMI1 and activating the Nrf2/ARE signaling pathway(2024/05/14)
作者:Zhimin Dou, Fei Lu, Jinjing Hu
期刊:Scientific Reports
DOI:10.1038/s41598-024-58248-8
影響因子 :4.600
引用產(chǎn)品:CL-0407
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RNA-seq transcriptome profiling of liver regeneration in mice identifies the miR-34b-5p/phosphoinositide-dependent protein kinase 1 axis as a potential target for hepatocyte proliferation(2022/08/21)
作者:Xiaohua Lei, Xiaoming Dai, Qian Wang
期刊:BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI:10.1016/j.bbrc.2022.08.049
影響因子 :3.100
引用產(chǎn)品:CL-0407
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Effects of protoscoleces excretory-secretory products of Echinococcus granulosus on hepatocyte growth, function, and glucose metabolism(2023/11/08)
作者:Guangyi Luo, Haiwen Li, Qiong Lu
期刊:ACTA TROPICA
DOI:10.1016/j.actatropica.2023.107066
影響因子 :2.700
引用產(chǎn)品:CL-0407
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Downregulated long non-coding RNA LINC01093 in liver fibrosis promotes hepatocyte apoptosis via increasing ubiquitination of SIRT1(2020/5/1)
作者:Tang Yinhe, Ma Naijing, Luo Hao
期刊:Journal Of Biochemistry
影響因子 :2.700
引用產(chǎn)品:CL-0407
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