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產(chǎn)品概述
名稱 | bEnd.3 [BEND3] (小鼠腦微血管內(nèi)皮細(xì)胞) (STR鑒定正確) |
別稱 | bEND.3; b.End3; Bend.3; bEnd3; brain-derived Endothelial cells.3 |
種屬 | 小鼠 |
生長特性 | 貼壁細(xì)胞 |
細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
凍存條件 | 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認(rèn)) |
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 1:2-1:4 |
推薦換液頻率 | 2-3次/周 |
注意事項(xiàng) | 1.該細(xì)胞在低密度下形態(tài)不規(guī)則,細(xì)胞數(shù)量少,鋪展面積大,高密度后形態(tài)會(huì)趨于一致,胞體密集;2.細(xì)胞增殖偏慢,初次傳代建議1:2,細(xì)胞穩(wěn)定后建議傳代比例也不超過1:4;3.注意觀察培養(yǎng)基顏色,顏色偏紫會(huì)導(dǎo)致細(xì)胞漂浮增加;4.消化時(shí)間會(huì)隨著細(xì)胞生長時(shí)間和細(xì)胞密度而有所不同,需要結(jié)合消化時(shí)細(xì)胞狀態(tài)來判斷,消化到細(xì)胞可以滑落方可終止。 |
背景描述 | The cells were transformed by infection with the NTKmT retrovirus vector that expresses polyomavirus middle T antigen |
年齡(性別) | 6周齡 |
組織來源 | 大腦皮層 |
細(xì)胞類型 | 轉(zhuǎn)化細(xì)胞系 |
生物安全等級(jí) | BSL-1 |
抗原表達(dá)情況 | ICAM-1 +; VCAM-1 +; MAdCAM-1 + |
基因表達(dá)情況 | von Willebrand factor,ICAM-1 +; VCAM-1 +; MAdCAM-1 +,The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL).,The expression of Peyer's Patch high endothelial receptor for lymphocytes, the mucosal vascular addressin (MAdCAM-1) and E-selectin can be induced on bEnd |
保藏機(jī)構(gòu) | ATCC; CRL-2299 BCRC; 60515 ECACC; 96091929 |
STR鑒定
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STR位點(diǎn)信息
18-3 18 6-7 11,12 5-5 14 X-1 26 1-2 13 7-1 29 8-1 16 1-1 10 19-2 13 15-3 19.3,20.3 12-1 17 6-4 17 4-2 18.3 3-2 14 2-1 9 13-1 16.2
11-2 14 17-2 12 -
STR鑒定圖
參考文獻(xiàn)
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