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EGF/Urogastrone/URG, Human, Recombinant

文獻(13) 說明書
一鍵復制產品信息
https://789.bio/ec/tDxaeP
  • https://789.bio/ec/tDxaeP

英文名稱：EGF/Urogastrone/URG, Human, Recombinant

貨號： PCK001

價格： ￥880 ￥680 ￥380 ￥180 ￥1080

規(guī)格：
500μg 100μg 50μg 10μg 1mg

產品信息

產品形態(tài) 粉末
產品規(guī)格 500μg / 100μg / 50μg / 10μg / 1mg
別名 Urogastrone;URG
物種 人
表達宿主 E.coli
序列信息 MNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR with polyhistidine tag at the C-terminus.
檢索號 P01133.2
分子量 7.16 kDa
表觀分子量 9-11 kDa
標簽 His-tag at the C-terminus
生物活性 His-tag at the C-terminus
純度 > 95% as determined by SDS-PAGE. Ni-NTA chromatography.
內毒素 < 0.1 EU per 1 μg of the protein by the LAL method.
儲存條件 Lyophilized protein should be stored at -5~-20℃ for 1 year. Upon reconstitution, store at 2-8℃ for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10% FBS, 5% HSA or 5% trehalose solution), protein aliquots should be stored at -5~-20℃ or -80℃ for 3-6 months.
運輸條件 Ambient temperature or ice pack.
有效期 12個月
制劑 The protein was lyophilized from a 0.2 μm filtered solution containing 1 × PBS, pH 8.0.
復融 It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 μg/mL. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
背景介紹 Epidermal growth factor (EGF) stimulates cell growth and differentiation by binding to its receptor, EGFR. Human EGF is a 6-kDa protein with 53 amino acid residues and three intramolecular disulfide bonds. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid. Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.

  • Q1:因子到貨了，里面有兩管溶液，一個是水，一個是海藻糖溶液，用哪一個稀釋呢？

    1、將裝有細胞因子的管子放入小離心機內短暫離心20~30s（離心機最大轉速即可），使附著在管壁的微量蛋白聚集在管底； 2、先用無菌水溶解因子，很少量水即可溶解因子，注意計算溶解后濃度不低于100 μg/mL； 3、用贈送的含海藻糖的PBS溶液進一步稀釋到您實驗合適的濃度即可，然后分裝后放入-20℃以下保藏； 4、因子復融重懸過程應輕柔，不可以震蕩渦旋。

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