FGF-2/bFGF/FGF-b (K128N), Human, Recombinant
英文名稱：FGF-2/bFGF/FGF-b (K128N), Human, Recombinant
貨號： PCK005
價格： ￥5080 ￥2180 ￥1180 ￥580 ￥7480
產(chǎn)品信息
| 產(chǎn)品形態(tài) | 液體 |
| 產(chǎn)品規(guī)格 | 500μg / 100μg / 50μg / 10μg / 1mg |
| 別名 | Fibroblast growth factor 2;FGF-2;Basic fibroblast growth factor;bFGF;Heparin-binding growth factor 2;HBGF-2;FGF2;FGFB |
| 物種 | 人 |
| 表達宿主 | E.coli |
| 序列信息 | Met1-Ser155 |
| 檢索號 | BAG70135.1 |
| 分子量 | 17.2kDa |
| 表觀分子量 | 17kDa |
| 生物活性 | Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 0.75 ng/mL. |
| 純度 | > 95% as determined by reducing SDS-PAGE. |
| 內(nèi)毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
| 儲存條件 | Store at ≤-70℃, stable for 6 months after receipt. Store at ≤-70℃, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| 運輸條件 | The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below. |
| 有效期 | 6個月 |
| 制劑 | Supplied as a 0.2 μm filtered solution of 20 mM Citrate, 10% Trehalose, 150 mM NaCl, 0.04% PS80, 0.5 mM EDTA, pH 5.5. |
| 背景介紹 | Fibroblast growth factors (FGF) are a family of heparin-binding secreted proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro, including mitogenesis, cellular migration, differentiation, angiogenesis, and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media, without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable, daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule, so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies, and maintained cell growth, pluripotency and differentiation potential with a 2-day feeding schedule. |
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期刊：CURRENT MOLECULAR MEDICINE
DOI：10.2174/1566524023666230324135330
影響因子：2.500
引用產(chǎn)品： EGF/Urogastrone/URG, Human, Recombinant, FGF-2/bFGF/FGF-b (K128N), Human, Recombinant
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期刊：APOPTOSIS
DOI：10.1007/s10495-022-01789-y
影響因子：5.600
引用產(chǎn)品： PC-9 細胞, A549 [A-549] 細胞, NCI-H1299 細胞, NCI-H1975 細胞, BEAS-2B 細胞, RPMI-1640 培養(yǎng)基, BEAS-2B細胞專用培養(yǎng)基, EGF/Urogastrone/URG, Human, Recombinant, FGF-2/ bFGF/ FGF-b (Q65I, C96S, N111G), Human, Recombinant
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