FGF-2/bFGF/FGF-b (Q65I, C96S, N111G), Human, Recombinant
英文名稱：FGF-2/bFGF/FGF-b (Q65I, C96S, N111G), Human, Recombinant
貨號(hào)： PCK006
價(jià)格： ￥5080 ￥1180 ￥580 ￥7480
產(chǎn)品信息
| 產(chǎn)品形態(tài) | 粉末 |
| 產(chǎn)品規(guī)格 | 500μg / 50μg / 10μg / 1mg |
| 別名 | Fibroblast Growth Factor 2;FGF-2;Basic fibroblast Growth Factor;bFGF;Heparin-binding Growth Factor 2;HBGF-2;FGF2;FGFB |
| 物種 | 人 |
| 表達(dá)宿主 | E.coli |
| 序列信息 | Met1-Ser155 |
| 檢索號(hào) | BAG70264.1 |
| 分子量 | 17.2 kDa |
| 表觀分子量 | 16 kDa |
| 純度 | > 95% as determined by reducing SDS-PAGE. |
| 內(nèi)毒素 | < 1.0 EU per 1 μg as determined by LAL test. |
| 儲(chǔ)存條件 | Lyophilized protein should be stored at -5~-20℃, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at -5~-20℃ for 3 months. |
| 運(yùn)輸條件 | Ambient temperature or ice pack. |
| 有效期 | 12個(gè)月 |
| 制劑 | Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 5% sucrose, 0.02% Tween 80, pH 8.0. |
| 復(fù)融 | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| 背景介紹 | Fibroblast Growth Factors (FGF) are a family of heparin-binding secreted Proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro, including mitogenesis, cellular migration, differentiation, angiogenesis, and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media, without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable, daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule, so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies, and maintained cell growth, pluripotency and differentiation potential with a 2-day feeding schedule. |
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期刊：CURRENT MOLECULAR MEDICINE
DOI：10.2174/1566524023666230324135330
影響因子：2.500
引用產(chǎn)品： EGF/Urogastrone/URG, Human, Recombinant, FGF-2/bFGF/FGF-b (K128N), Human, Recombinant
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期刊：APOPTOSIS
DOI：10.1007/s10495-022-01789-y
影響因子：5.600
引用產(chǎn)品： PC-9 細(xì)胞, A549 [A-549] 細(xì)胞, NCI-H1299 細(xì)胞, NCI-H1975 細(xì)胞, BEAS-2B 細(xì)胞, RPMI-1640 培養(yǎng)基, BEAS-2B細(xì)胞專用培養(yǎng)基, EGF/Urogastrone/URG, Human, Recombinant, FGF-2/ bFGF/ FGF-b (Q65I, C96S, N111G), Human, Recombinant
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