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![BT-474 [BT474] (人乳腺導(dǎo)管癌細(xì)胞) (STR鑒定正確)](/Public/upfile/pic/xbx/CL-0040-1.jpg)
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BT-474 [BT474] (人乳腺導(dǎo)管癌細(xì)胞) (STR鑒定正確)
貨號(hào)：CL-0040
規(guī)格：1×10?cells/T25培養(yǎng)瓶
價(jià)格： ￥1600
生長(zhǎng)培養(yǎng)基：RPMI-1640＋10μg/ml Insulin＋2mM L-glutamine＋20% FBS＋1% P/S
產(chǎn)品概述
| 名稱 | BT-474 [BT474] (人乳腺導(dǎo)管癌細(xì)胞) (STR鑒定正確) |
| 別稱 | Bt-474; BT474 |
| 種屬 | 人 |
| 生長(zhǎng)特性 | 貼壁細(xì)胞 |
| 細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認(rèn)） |
生長(zhǎng)培養(yǎng)基:
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
|
| 推薦傳代比例 | 1:2-1:4 |
| 推薦換液頻率 | 2-3次/周 |
| 注意事項(xiàng) | 1.BT-474細(xì)胞生長(zhǎng)緩慢，細(xì)胞主要是成片生長(zhǎng)；2.匯合至80%即可傳代，細(xì)胞不會(huì)長(zhǎng)太滿，5-6天傳代一次。 |
| 背景描述 | BT-474 [BT474]細(xì)胞是由E·Lasfargues和W·G·Coutinho從一個(gè)實(shí)心的乳腺浸潤(rùn)性導(dǎo)管癌中分離到的細(xì)胞株。 |
| 年齡（性別） | 女性；60歲 |
| 組織來(lái)源 | 乳腺導(dǎo)管癌 |
| 細(xì)胞類型 | 腫瘤細(xì)胞 |
| 腫瘤類型 | 乳腺癌細(xì)胞 |
| 生物安全等級(jí) | BSL-1 |
| 倍增時(shí)間 | ~84-100 hours |
| 致瘤性 | Yes, in Amsterdam/IMR rats with regression in 10 days.Yes, in nude mice. |
| 保藏機(jī)構(gòu) | ATCC; CRL-7913ATCC; HTB-20 BCRC; 60359 BCRJ; 0353 DSMZ; ACC-64 |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X CSF1PO 10,11 D2S1338 19 D3S1358 17 D5S818 11,13 D7S820 9,12 D8S1179 10,12 D13S317 11 D16S539 9,11 D18S51 13,18 D19S433 14,17 D21S11 28,32.2 FGA 22,25 PentaD 9,14 PentaE 5 TH01 7 TPOX 8 vWA 15,16 D6S1043 12,17 D12S391 17,18 D2S441 10,14 -
STR鑒定圖
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參考文獻(xiàn)
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Development of a rare earth nanoprobe enables in vivo real-time detection of sentinel lymph node metastasis of breast cancer using NIR-IIb imaging(2023/08/04)
作者：Yuanyuan Zhu, Liang Song, Yongqu Zhang
期刊：CANCER RESEARCH
DOI：10.1158/0008-5472.CAN-22-3432
影響因子 ：11.200
引用產(chǎn)品：CL-0007 CL-0040 CL-0041 CL-0149 CL-0211 CL-0228 CL-0525
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Long-term cadmium exposure induces epithelial-mesenchymal transition in breast cancer cells by activating CYP1B1-mediated glutamine metabolic reprogramming in BT474 cells and MMTV-Erbb2 mice(2024/02/07)
作者：Jingdian Li, Peng Gao, Mingke Qin
期刊：SCIENCE OF THE TOTAL ENVIRONMENT
DOI：10.1016/j.scitotenv.2024.170773
影響因子 ：9.800
引用產(chǎn)品：CL-0040
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Up-regulated circBACH2 contributes to cell proliferation, invasion, and migration of triple-negative breast cancer(2021/04/19)
作者：Xinxing Wang, Bingjian Xue, Yujie Zhang
期刊：Cell Death & Disease
DOI：10.1038/s41419-021-03684-x
影響因子 ：9.000
引用產(chǎn)品：CL-0040 CL-0041 CL-0149 CL-0150 CL-0152 CL-0290 CL-0525
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CircEGFR reduces the sensitivity of pirarubicin and regulates the malignant progression of triple-negative breast cancer via the miR-1299/EGFR axis(2023/06/09)
作者：Jiulong Ma, Chen Chen, Zhimin Fan
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2023.125295
影響因子 ：8.200
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UCHL1 contributes to insensitivity to endocrine therapy in triple-negative breast cancer by deubiquitinating and stabilizing KLF5(2024/03/11)
作者：Juan Li, Yu Liang, Shijie Zhou
期刊：BREAST CANCER RESEARCH
DOI：10.1186/s13058-024-01800-1
影響因子 ：7.400
引用產(chǎn)品：CL-0040
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A Novel Optical Fiber Terahertz Biosensor Based on Anti-Resonance for The Rapid and Nondestructive Detection of Tumor Cells(2023/10/23)
作者：Zhe He, Yueping Luo, Guorong Huang
期刊：Biosensors-Basel
影響因子 ：5.400
引用產(chǎn)品：CL-0040
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TILRR (FREM1 isoform 2) is a prognostic biomarker correlated with immune infiltration in breast cancer(2020/10/08)
作者：Xiaoyi Xu, Wenjing Guo, Shihua Pan
期刊：Aging-US
影響因子 ：5.200
引用產(chǎn)品：CL-0040
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Constructing a novel prognostic model for triple-negative breast cancer based on genes associated with vasculogenic mimicry(2024/05/08)
作者：Yu Ren, Luyi Feng, Zhihua Tan
期刊：Aging-US
影響因子 ：5.200
引用產(chǎn)品：CL-0040
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A comprehensive analysis of the role of QPRT in breast cancer(2023/09/18)
作者：Yiqing Yan, Lun Li, Zixin Wang
期刊：Scientific Reports
DOI：10.1038/s41598-023-42566-4
影響因子 ：4.600
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TTK inhibition activates STING signal and promotes anti-PD1 immunotherapy in breast cancer(2023/12/18)
作者：Xiang Hu, Guangmei Li, Sisi Li
期刊：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI：10.1016/j.bbrc.2023.149388
影響因子 ：3.100
引用產(chǎn)品：CL-0040
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Analysis of TLR2 in Primary Endocrine Resistant of Breast Cancer(2024/02/22)
作者：Yunmei Wang, Guangxi Wang, Xiang Wang
期刊：Frontiers in Bioscience-Landmark
影響因子 ：3.100
引用產(chǎn)品：CL-0040
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Deciphering the map of METTL14-mediated lncRNA m6A modification at the transcriptome-wide level in breast cancer(2022/11/06)
作者：Dandan Yi, Fazhan Xu, Ru Wang, Chaoyu Jiang, Jiabo Qin, YiHsuan Lee, Xianbiao Shi, Jianfeng Sang
期刊：JOURNAL OF CLINICAL LABORATORY ANALYSIS
影響因子 ：2.700
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