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![SK-BR-3 [SKBR3] (人乳腺腺癌細(xì)胞) (STR鑒定正確)](/Public/upfile/pic/xbx/CL-0211-1.jpg)
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SK-BR-3 [SKBR3] (人乳腺腺癌細(xì)胞) (STR鑒定正確)
貨號(hào)：CL-0211
規(guī)格：1×10?cells/T25培養(yǎng)瓶
價(jià)格： ￥1600
生長培養(yǎng)基：McCoy’s 5A＋10% FBS＋1% P/S
產(chǎn)品概述
| 名稱 | SK-BR-3 [SKBR3] (人乳腺腺癌細(xì)胞) (STR鑒定正確) |
| 別稱 | SK-Br-3; Sk-Br-3; SK BR 03; SKBR-3; SKBr-3; SK-BR3; SKBr3; SkBr3; SKBR3 |
| 種屬 | 人 |
| 生長特性 | 貼壁細(xì)胞 |
| 細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認(rèn)） |
生長培養(yǎng)基:
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
|
| 推薦傳代比例 | 1:2-1:4 |
| 推薦換液頻率 | 2-3次/周 |
| 注意事項(xiàng) | 1. SK-BR-3細(xì)胞從冷凍保存中恢復(fù)的速度可能很慢。SK-BR-3細(xì)胞在低溫保存恢復(fù)過程中的附著會(huì)很輕。這很正常。這種細(xì)胞系在培養(yǎng)的整個(gè)第一周以及每次繼代培養(yǎng)后都表現(xiàn)出松散的附著和漂浮的細(xì)胞，這并不少見；2. 如果存在大量漂浮物，尤其是在生長的第一周，在啟動(dòng)復(fù)蘇后幾天內(nèi)保持細(xì)胞不受干擾。如果仍然有很多漂浮物，用臺(tái)盼藍(lán)檢查生存能力。通過輕輕離心保留漂浮細(xì)胞，并在更換培養(yǎng)基時(shí)將其與附著的細(xì)胞一起添加回同一培養(yǎng)容器中，這一點(diǎn)很重要。不要分離或丟棄漂浮細(xì)胞。細(xì)胞最初以小斑塊或細(xì)胞群的形式附著，許多細(xì)胞團(tuán)仍處于懸浮狀態(tài)。幾天后，生長將從貼壁細(xì)胞群向外延伸。通常也會(huì)出現(xiàn)一些細(xì)胞碎片。漂浮細(xì)胞應(yīng)始終通過溫和離心（125xg，持續(xù)5-7min）保留，并在換液或傳代后放回培養(yǎng)容器；3. SK-BR-3細(xì)胞傾向于相互堆積。在細(xì)胞達(dá)到70-80%匯合度之前不要用胰酶消化處理細(xì)胞。如果讓細(xì)胞過度生長，細(xì)胞就會(huì)分離漂浮。 |
| 背景描述 | SK-BR-3 [SKBR3]細(xì)胞是由Trempe·G和Old·L·J于1970年從一位43歲的白人女性乳腺癌患者的胸腔積液中分離得到的。SK-BR-3 [SKBR3]細(xì)胞亞顯微結(jié)構(gòu)特征包括微絲和橋粒、肝糖原顆粒、大溶酶體、成束的細(xì)胞質(zhì)纖絲；SK-BR-3 [SKBR3]細(xì)胞過表達(dá)HER2/c-erb-2基因產(chǎn)物。 |
| 年齡（性別） | 女性；43歲 |
| 組織來源 | 乳腺；乳房；肋膜滲出液轉(zhuǎn)移灶 |
| 細(xì)胞類型 | 腫瘤細(xì)胞 |
| 腫瘤類型 | 乳腺癌細(xì)胞 |
| 生物安全等級(jí) | BSL-1 |
| 倍增時(shí)間 | ~48-72 hours |
| 致瘤性 | Yes, in nude mice; forms poorly differentiated adenocarcinoma. |
| 受體表達(dá)情況 | Blood Type A; Rh+; HLA A11, Bw22 (+/-), B40, B18 |
| 保藏機(jī)構(gòu) | ATCC; HTB-30 DSMZ; ACC-736 |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X CSF1PO 12 D2S1338 20,25 D3S1358 17 D5S818 9,12 D7S820 9,12 D8S1179 11,12 D13S317 11,12 D16S539 9 D18S51 10,13 D19S433 14 D21S11 30,30.2 FGA 20 PentaD 9,12 PentaE 10,11 TH01 8,9 TPOX 8,11 vWA 17 D6S1043 11,19 D12S391 18,20 D2S441 13,14 -
STR鑒定圖
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參考文獻(xiàn)
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