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SV-HUC-1 (人輸尿管上皮永生化細(xì)胞) (STR鑒定正確)
貨號(hào):CL-0222
規(guī)格:1×10?cells/T25培養(yǎng)瓶
價(jià)格: ¥1600
生長(zhǎng)培養(yǎng)基:Ham's F-12K+10% FBS+1% P/S
產(chǎn)品概述
名稱 | SV-HUC-1 (人輸尿管上皮永生化細(xì)胞) (STR鑒定正確) |
別稱 | HUC-1; SV-HUC; SVHUC |
種屬 | 人 |
生長(zhǎng)特性 | 貼壁細(xì)胞 |
細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
凍存條件 | 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認(rèn)) |
生長(zhǎng)培養(yǎng)基:
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 1:2-1:4 |
推薦換液頻率 | 2-3次/周 |
注意事項(xiàng) | 該細(xì)胞較難消化,注意延長(zhǎng)消化時(shí)間,消化到細(xì)胞收縮變圓,輕拍培養(yǎng)瓶側(cè)邊,細(xì)胞可以滑落方可終止消化。 |
背景描述 | SV-HUC-1細(xì)胞是正常輸尿管組織用SV40病毒轉(zhuǎn)染建株的。反復(fù)用非洲綠猴腎細(xì)胞平板測(cè)試檢測(cè)傳染性SV40的生成,結(jié)果都呈陰性;SV-HUC-1細(xì)胞在脅迫(如曝露于化學(xué)試劑)下可能激活病毒。 |
年齡(性別) | 男性;11歲 |
組織來(lái)源 | 輸尿管,輸尿管上皮 |
細(xì)胞類型 | 轉(zhuǎn)化細(xì)胞系 |
生物安全等級(jí) | BSL-2 |
致瘤性 | No, nude mice. |
基因表達(dá)情況 | uroepithelial keratins; SV40 T antigen |
保藏機(jī)構(gòu) | ATCC; CRL-9520 |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X,Y CSF1PO 10,11 D2S1338 17 D3S1358 14,17 D5S818 12,14 D7S820 10,11 D8S1179 14,16 D13S317 12,13 D16S539 11 D18S51 15,16 D19S433 14 D21S11 29,30 FGA 21,22 PentaD 13 PentaE 10,20 TH01 9,9.3 TPOX 8,10 vWA 14 D6S1043 11,13 D12S391 18 D2S441 10,13 -
STR鑒定圖
參考文獻(xiàn)
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