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產(chǎn)品概述
Leibovitz's L-15培養(yǎng)基的緩沖系統(tǒng)由磷酸鹽和游離堿基氨基酸組成，代替了傳統(tǒng)的碳酸氫鈉緩沖系統(tǒng)，同時(shí)，用半乳糖和丙酮酸鈉代替葡萄糖可以防止酸性代謝副產(chǎn)物的形成，有助于維持培養(yǎng)液PH的穩(wěn)定，適合用于非CO2平衡環(huán)境的細(xì)胞培養(yǎng)。Leibovitz's L-15培養(yǎng)基適用于猴腎細(xì)胞和HEP-2的培養(yǎng)、原代（如胚胎和成人組織）細(xì)胞分離、多種病毒的培養(yǎng)以及神經(jīng)元的培養(yǎng)等。
本產(chǎn)品含有多類細(xì)胞培養(yǎng)所需的氨基酸、維生素、無機(jī)鹽等多種成分，但不含蛋白質(zhì)、脂類或任何生長因子，故此產(chǎn)品需搭配血清或無血清添加物使用。
成分說明
形態(tài) | 液體 |
濃度 | 1× |
規(guī)格 | 500mL |
PH | 7.2～7.4 |
緩沖系統(tǒng) | 磷酸鹽、氨基酸緩沖系統(tǒng) |
L-谷氨酰胺 | 300mg/L |
NaHCO3 | 無 |
D-葡萄糖 | 無 |
D-半乳糖 | 900mg/L |
丙酮酸鈉 | 550mg/L |
HEPES緩沖劑 | 無 |
酚紅指示劑 | 10mg/L |
儲(chǔ)存條件 | 2～8℃，避光 |
運(yùn)輸條件 | 常溫 |
有效期 | 18個(gè)月 |
注意事項(xiàng)
1、本產(chǎn)品經(jīng)過濾除菌，使用時(shí)應(yīng)注意無菌操作，避免污染；
2、本品不適用于CO2環(huán)境，CO2會(huì)導(dǎo)致培養(yǎng)液PH降低（培養(yǎng)液變黃），嚴(yán)重影響細(xì)胞生長；
3、為保持本產(chǎn)品的最佳使用效果，請(qǐng)勿進(jìn)行凍融處理；
4、本產(chǎn)品僅用于科研或進(jìn)一步研究使用，不用于診斷和治療。
參考文獻(xiàn)
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In vivo imaging of mitochondrial DNA mutations using an integrated nano Cas12a sensor(2023/11/24)
作者：Yanan Li, Yonghua Wu, Ru Xu
期刊：Nature Communications
DOI：10.1038/s41467-023-43552-0
影響因子 ：16.600
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作者：Yan Guo, Yuqing Cui, Yangyang Li
期刊：Cancer Communications
影響因子 ：16.200
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Circ-MBOAT2 knockdown represses tumor progression and glutamine catabolism by miR-433-3p/GOT1 axis in pancreatic cancer(2021/04/08)
作者：Zhou Xiaoxiao, Liu Kun, Cui Jing
期刊：Journal of Experimental & Clinical Cancer Research
DOI：10.1186/s13046-021-01894-x
影響因子 ：11.300
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The perturbation of parabens on the neuroendocrine system in zebrafish larvae(2023/04/21)
作者：Jiefeng Liang, Xiaoxi Yang, Tongtong Xiang
期刊：SCIENCE OF THE TOTAL ENVIRONMENT
DOI：10.1016/j.scitotenv.2023.163593
影響因子 ：9.800
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WD repeat protein 54-mediator of ErbB2-driven cell motility 1 axis promotes bladder cancer tumorigenesis and metastasis and impairs chemosensitivity(2023/01/07)
作者：Xiaosong Wei, Beibei Wang, Zixin Wu
期刊：CANCER LETTERS
DOI：10.1016/j.canlet.2023.216058
影響因子 ：9.700
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作者：Ying Zhang, Changjun Yin, Chao Wei, Shuai Xia, Zhu Qiao, Xue-Wen Zhang, Bin Yu, Jun Zhou, Rong Wang
期刊：PHARMACOLOGICAL RESEARCH
DOI：10.1016/j.phrs.2022.106534
影響因子 ：9.300
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作者：Xinli Liu, Ge Zhang, Tongyao Yu
期刊：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
DOI：10.1016/j.ijbiomac.2023.126147
影響因子 ：8.200
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作者：Jia Ma, Wenyang Zhou, Yifeng Yuan
期刊：Cancer Gene Therapy
DOI：10.1038/s41417-023-00609-y
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An antihypertensive drug-AT1 inhibitor attenuated BRCA development promoted by chronic psychological stress via Ang II/PARP1/FN1 pathway(2024/01/20)
作者：Yuqing Cui, Ming Zhuang, Zheping Huang
期刊：BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
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期刊：BIOCHEMICAL PHARMACOLOGY
影響因子 ：5.800
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作者：Ziyue Jia, Xintong Yang, Huiyou Chen
期刊：Frontiers in Pharmacology
DOI：10.3389/fphar.2024.1347970
影響因子 ：5.600
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期刊：Bioengineered
DOI：10.1080/21655979.2021.1924544
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Transcriptomic Analysis Revealed the Regulatory Mechanisms of Oocyte Maturation and Hydration in Orange-Spotted Grouper (Epinephelus coioides)(2019/05/25)
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microRNA-4429-5p suppresses the malignant development of colon cancer by targeting matrix metalloproteinase 16(2021/08/26)
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影響因子 ：2.600
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影響因子 ：2.300
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影響因子 ：2.100
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期刊：BIOLOGICAL & PHARMACEUTICAL BULLETIN
影響因子 ：2.000
引用產(chǎn)品：PM151010
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