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SW620 (人結(jié)腸癌細胞) (STR鑒定正確)
貨號:CL-0225
規(guī)格:1×10?cells/T25培養(yǎng)瓶
價格: ¥1600
生長培養(yǎng)基:Leibovitz's L-15+10% FBS+1% P/S
產(chǎn)品概述
名稱 | SW620 (人結(jié)腸癌細胞) (STR鑒定正確) |
別稱 | SW-620; SW 620; SW.620 |
種屬 | 人類 |
生長特性 | 貼壁細胞 |
細胞形態(tài) | 上皮細胞樣 |
凍存條件 | 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度:液氮 |
培養(yǎng)方案A(默認) |
生長培養(yǎng)基:
培養(yǎng)條件:
氣相:空氣,100%, 溫度:37℃
|
培養(yǎng)方案B(可選) |
培養(yǎng)條件:
氣相:空氣,95%;CO2,5%, 溫度:37℃
|
推薦傳代比例 | 1:3-1:4 |
推薦換液頻率 | 2-3次/周 |
注意事項 | 1.該細胞推薦使用Leibovitz's L-15培養(yǎng)基進行培養(yǎng),Leibovitz's L-15不可以通入二氧化碳,會產(chǎn)生細胞毒性;2.如您沒有無二氧化碳的培養(yǎng)箱,可使用DMEM替代Leibovitz's L-15,使用DMEM培養(yǎng)基時即可正常通入5%二氧化碳;3.配套專用培養(yǎng)基默認Leibovitz's L-15配置,如需DMEM配方,請聯(lián)系銷售下單備注更改。 |
背景描述 | SW620細胞是從一個51歲男性白人組織中分離得到的,由A·Leibovitz等從一個淋巴結(jié)建株。SW620細胞主要由無絨毛的小園球細胞和雙極細胞組成;SW620細胞僅合成少量癌胚抗原(CEA),在裸鼠中有高度的致瘤性。 |
年齡(性別) | 男性;51歲 |
組織來源 | 結(jié)直腸腺癌,來自轉(zhuǎn)移淋巴結(jié) |
細胞類型 | 腫瘤細胞 |
腫瘤類型 | 腸癌細胞 |
生物安全等級 | BSL-1 |
倍增時間 | ~20-26 hours |
致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells). |
抗原表達情況 | Blood Type A; Rh+ |
基因表達情況 | Carcinoembryonic antigen (CEA) 0.15 ng/10^6 cells/10 days; transforming growth factor alpha; matrilysin. The cells are negative for expression of CSAp (CSAp-) and colon antigen 3, negative. The cells are positive for keratin by immunoperoxidase staining. The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes. |
保藏機構(gòu) | ATCC; CCL-227 ECACC; 87051203 |
STR鑒定
-
STR位點信息
Amelogenin X CSF1PO 13,14 D2S1338 17,24 D3S1358 16 D5S818 13 D7S820 8,9 D8S1179 13 D13S317 12 D16S539 9,13 D18S51 13 D19S433 13 D21S11 30,30.2 FGA 24 PentaD 9,15 PentaE 10 TH01 8 TPOX 11 vWA 16 D6S1043 11,12 D12S391 17 D2S441 10,15 -
STR鑒定圖
參考文獻
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