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RKO (人結(jié)腸腺癌細(xì)胞) (STR鑒定正確)
貨號(hào)：CL-0196
規(guī)格：1×10?cells/T25培養(yǎng)瓶
價(jià)格： ￥1500
生長培養(yǎng)基：MEM（含NEAA）＋10% FBS＋1% P/S
產(chǎn)品概述
| 名稱 | RKO (人結(jié)腸腺癌細(xì)胞) (STR鑒定正確) |
| 種屬 | 人 |
| 生長特性 | 貼壁細(xì)胞 |
| 細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認(rèn)） |
生長培養(yǎng)基:
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
|
| 推薦傳代比例 | 1:3-1:4 |
| 推薦換液頻率 | 2-3次/周 |
| 注意事項(xiàng) | 該細(xì)胞對(duì)溫度變化敏感，培養(yǎng)時(shí)使用的培養(yǎng)基需預(yù)熱，溫度較低的培養(yǎng)基使用會(huì)導(dǎo)致變圓收縮；傳代后到細(xì)胞完全伸展開需要72h以上。 |
| 背景描述 | RKO細(xì)胞是一個(gè)低分化的結(jié)腸癌細(xì)胞系。RKO細(xì)胞含有野生型p53，但缺乏人甲狀腺受體核受體(h-TRβ1)。RKO細(xì)胞的p53蛋白的水平高于RKO-E6細(xì)胞，RKO細(xì)胞是RKO-E6細(xì)胞和RKO-AS45-1細(xì)胞的親本細(xì)胞系。RKO細(xì)胞在裸鼠中成瘤，且在軟瓊脂中形成集落。 |
| 組織來源 | 結(jié)腸癌 |
| 細(xì)胞類型 | 腫瘤細(xì)胞 |
| 腫瘤類型 | 腸癌細(xì)胞 |
| 生物安全等級(jí) | BSL-1 |
| 倍增時(shí)間 | ~21 hours |
| 致瘤性 | Yes, in nude mice.Yes, in soft agar. |
| 受體表達(dá)情況 | urokinase receptor (u-PAR) |
| 保藏機(jī)構(gòu) | ATCC; CRL-2577 |
STR鑒定
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STR位點(diǎn)信息
Amelogenin X CSF1PO 8,10 D2S1338 16 D3S1358 16,19 D5S818 11,13 D7S820 8,10 D8S1179 9,13,14 D13S317 8,11 D16S539 12,13 D18S51 11,12 D19S433 14 D21S11 27,30 FGA 20,21,22,23 PentaD 10,11 PentaE 11,13 TH01 6,10 TPOX 11 vWA 16,22 D6S1043 14.1,19 D12S391 15,20 D2S441 11 -
STR鑒定圖
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參考文獻(xiàn)
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Therapy-induced senescent tumor cell-derived extracellular vesicles promote colorectal cancer progression through SERPINE1-mediated NF-κB p65 nuclear translocation(2024/04/04)
作者：Dan Zhang, Jianwei Zhang, Hui Xu
期刊：Molecular Cancer
DOI：10.1186/s12943-024-01985-1
影響因子 ：37.300
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Mitochondrial genome transfer drives metabolic reprogramming in adjacent colonic epithelial cells promoting TGFβ1-mediated tumor progression(2024/04/30)
作者：Bingjie Guan, Youdong Liu, Bowen Xie
期刊：Nature Communications
DOI：10.1038/s41467-024-48100-y
影響因子 ：16.600
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Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis(2024/01/17)
作者：Can Cheng, Hanhui Yao, Heng Li
期刊：Acta Pharmaceutica Sinica B
DOI：10.1016/j.apsb.2024.01.006
影響因子 ：14.500
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Acetyltransferase NAT10 regulates the Wnt/β-catenin signaling pathway to promote colorectal cancer progression via ac4C acetylation of KIF23 mRNA(2022/12/15)
作者：Chi Jin, Tuo Wang, Dongsheng Zhang
期刊：JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
DOI：10.1186/s13046-022-02551-7
影響因子 ：11.300
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Suppression of Tumor or Host Intrinsic CMTM6 Drives Antitumor Cytotoxicity in a PD-L1–Independent Manner(2023/02/03)
作者：Yiru Long, Runqiu Chen, Xiaolu Yu
期刊：Cancer Immunology Research
DOI：10.1158/2326-6066.CIR-22-0439
影響因子 ：10.100
引用產(chǎn)品：CL-0005 CL-0007 CL-0019 CL-0071 CL-0105 CL-0129 CL-0140 CL-0196 CL-0246 CL-0319
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Enhancing tumor-specific recognition of programmable synthetic bacterial consortium for precision therapy of colorectal cancer(2024/01/20)
作者：Tuoyu Zhou, Jingyuan Wu, Haibo Tang
期刊：npj Biofilms and Microbiomes
DOI：10.1038/s41522-024-00479-8
影響因子 ：9.200
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PD-1-siRNA Delivered by Attenuated Salmonella Enhances the Antitumor Effect of Chloroquine in Colon Cancer(2021/07/06)
作者：Shuya Lu, Jianhui Gao, Huijie Jia
期刊：Frontiers in Immunology
影響因子 ：7.300
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LncRNA-ENST00000543604 exerts a tumor-promoting effect via miRNA 564/AEG-1 or ZNF326/EMT and predicts the prognosis of and chemotherapeutic effect in colorectal cancer.(2022/08/23)
作者：Weimin Wang, Zhen Zhou, Xiaojun Dai
期刊：Frontiers in Oncology
影響因子 ：5.738
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Attenuated Salmonella-delivered PD-1 siRNA enhances the antitumor effects of EZH2 inhibitors in colorectal cancer(2023/09/12)
作者：Mengmeng Lv, Sheng Guo, Xinyu Zhang
期刊：INTERNATIONAL IMMUNOPHARMACOLOGY
DOI：10.1016/j.intimp.2023.110918
影響因子 ：5.600
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m6A methyltransferase METTL16 mediates immune evasion of colorectal cancer cells via epigenetically regulating PD-L1 expression(2023/08/29)
作者：Ailei Wang, Yingjie Sun, Xince Wang
期刊：Aging-US
影響因子 ：5.200
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Lycorine Induces autophagy-associated apoptosis by targeting MEK2 and enhances vemurafenib activity in colorectal cancer(2020/01/03)
作者：Man Hu, Zhaomin Yu, Peiyuan Mei
期刊：Aging-US
影響因子 ：5.200
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Notum palmitoleoyl-protein carboxylesterase regulates Fas cell surface death receptor-mediated apoptosis via the Wnt signaling pathway in colon adenocarcinoma(2021/8/17)
作者：Hua Gong, Qiang Niu, Yi Zhou
期刊：Bioengineered
DOI：10.1080/21655979.2021.1961657
影響因子 ：4.900
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Fluorescent Quinolinium Derivative as Novel Mitochondria Probe and Function Modulator by Targeting Mitochondrial RNA(2023/03/16)
作者：Bozheng Wang, Yingchen Zhou, Yuwei Lin
期刊：MOLECULES
影響因子 ：4.600
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Screening and validation of the optimal panel of reference genes in colonic epithelium and relative cancer cell lines(2023/10/18)
作者：Yang Hu, Qi Jiang, Xiang Zhai
期刊：Scientific Reports
DOI：10.1038/s41598-023-45174-4
影響因子 ：4.600
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Variation of Saponins in Sanguisorba officinalis L. before and after Processing (Paozhi) and Its Effects on Colon Cancer Cells In Vitro(2022/12/19)
作者：Zhengyang Wang, Chunjuan Yang, Lihong Wu
期刊：MOLECULES
影響因子 ：4.600
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EGCG Enhances the Chemosensitivity of Colorectal Cancer to Irinotecan through GRP78-MediatedEndoplasmic Reticulum Stress.(2022/09/13)
作者：Wenbing Wu, Hui Gou, Bin Xiang
期刊：Journal of Oncology
影響因子 ：4.501
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Overexpression of ZNF169 promotes the growth and proliferation of colorectal cancer cells via the upregulation of ANKZF1(2024/04/23)
作者：Jie Zhang, Ye Wang, Shiyang Hou
期刊：ONCOLOGY REPORTS
影響因子 ：4.200
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Three New Compounds, Licopyranol A–C, Together with Eighteen Known Compounds Isolated from Glycyrrhiza glabra L. and Their Antitumor Activities(2022/09/23)
作者：Shanshan Wang, Jameel Hizam Alafifi, Qin Chen
期刊：Metabolites
影響因子 ：4.100
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CILP2: A prognostic biomarker associated with immune infiltration in colorectal cancer.(2023/04/20)
作者：Xueli Wang, Yu Zhang, Niping Song
期刊：Heliyon
DOI：10.1016/j.heliyon.2023.e15535
影響因子 ：4.000
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10-Secocycloartane (=9,19-cyclo-9,10-secolanostane) triterpenoid saponins: Huangqiyenins M–X from Astragalus membranaceus (Fisch.) Bge(2024/03/30)
作者：Mengqi Yan, Feng Xu, Haixue Kuang
期刊：PHYTOCHEMISTRY
DOI：10.1016/j.phytochem.2024.114072
影響因子 ：3.800
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The bromodomain and extra-terminal domain inhibitor JQ1 synergistically sensitizes human colorectal cancer cells to topoisomerase I inhibitors through repression of Mre11-mediated DNA repair pathway(2020/09/26)
作者：Linping Lei, Xuqin Xie, Long He
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DOI：10.1007/s10637-020-01014-0
影響因子 ：3.400
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Methyltransferase like 3 enhances pinin mRNA stability through N6-methyladenosine modification to augment tumourigenesis of colon adenocarcinoma(2022/09/07)
作者：Min He, Danling Jiang, Anying Xun
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DOI：10.1113/EP090273
影響因子 ：2.700
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TRIM29 knockdown prevented the colon cancer progression through decreasing the ubiquitination levels of KRT5(2023/08/30)
作者：Lihui Sun, Dawei Wang, Zhenyu Chen
期刊：Open Life Sciences
影響因子 ：2.200
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Exosomes-derived miR-548am-5p promotes colorectal cancer progression(2023/11/30)
作者：Fangfang Li, Minglei Zhang, Xiaodong Yin
期刊：CELLULAR AND MOLECULAR BIOLOGY
DOI：10.14715/cmb/2023.69.12.17
影響因子 ：1.600
引用產(chǎn)品：CL-0196
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產(chǎn)品資料
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