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產(chǎn)品概述
| 名稱 | DLD-1 (人結(jié)直腸腺癌上皮細(xì)胞) (STR鑒定正確) |
| 別稱 | DLD 1; DLD1; CoCL3 |
| 種屬 | 人 |
| 生長特性 | 貼壁細(xì)胞 |
| 細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
| 凍存條件 | 凍存液：55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO 溫度：液氮 |
| 培養(yǎng)方案A（默認(rèn)） |
培養(yǎng)條件:
氣相：空氣，95%；CO2，5%, 溫度：37℃
|
| 推薦傳代比例 | 1:3-1:4 |
| 推薦換液頻率 | 2-3次/周 |
| 背景描述 | DLD-1細(xì)胞是由D·L·Dexter和其同事于1977-1979年分離的兩株結(jié)直腸腺癌細(xì)胞株中的一株。在ATCC和其它地方進(jìn)行的DNA指紋鑒定和染色體組型分析表明DLD-1細(xì)胞與HCT-15細(xì)胞相似，說明這兩者是來自同一個人的不同克隆。DLD-1細(xì)胞和HCT-15細(xì)胞的遺傳起源可通過DNA指紋鑒定證實，但染色體組型分析顯示它們?nèi)狈θ旧w標(biāo)記一致改變或數(shù)目上一致改變。DLD-1細(xì)胞的CSAp陰性(CSAp-)，p53抗原表達(dá)呈陽性(p53抗原產(chǎn)生了一個C→T點突變導(dǎo)致241位的Ser→Phe)。DLD-1細(xì)胞角蛋白免疫過氧化物酶染色陽性，癌基因c-myc、K-ras、H-ras、N-ras、myb、sis和fos的表達(dá)呈陽性，癌基因N-myc的表達(dá)未做檢測。DLD-1細(xì)胞表達(dá)腫瘤特異性核基質(zhì)蛋白CC-2、CC-3、CC-4、CC-5和CC-6。1979年提交到ATCC的DLD-1細(xì)胞代數(shù)不明且污染了支原體，其后經(jīng)過12周多種抗生素聯(lián)合培養(yǎng)處理，處理之后每周用Hoechst染色和標(biāo)準(zhǔn)培養(yǎng)法檢測。其后連續(xù)11個月不加抗生素培養(yǎng)，DLD-1細(xì)胞所有的檢測呈陰性。 |
| 年齡（性別） | 男性；成人 |
| 組織來源 | 結(jié)直腸腺癌上皮細(xì)胞 |
| 細(xì)胞類型 | 腫瘤細(xì)胞 |
| 腫瘤類型 | 腸癌細(xì)胞 |
| 生物安全等級 | BSL-1 |
| 倍增時間 | ~24-48 hours |
| 致瘤性 | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1×10^7 cells). |
| 抗原表達(dá)情況 | Blood Type O.The cells are weakly positive for keratins and vimentin.The cells are positive for keratin by immunoperoxidase staining.DLD-1 cells are positive for p53 antigen expression (the p53 antigen produced has a C -> T |
| 基因表達(dá)情況 | carcinoembryonic antigen (CEA) 0.5 ng/10^6 cells/10 days; colon antigen 3. |
| 保藏機(jī)構(gòu) | ATCC; CCL-221 BCRC; 60132 DSMZ; ACC-278 ECACC; 90102540 |
STR鑒定
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STR位點信息
Amelogenin X,Y CSF1PO 11,12 D2S1338 17,25 D3S1358 17 D5S818 13 D7S820 10,12 D8S1179 15 D13S317 8,11 D16S539 12,13 D18S51 11,17 D19S433 14,16 D21S11 29,32.2 FGA 22 PentaD 9,14 PentaE 7,14 TH01 7,9.3 TPOX 8,11 vWA 18,19 D6S1043 11,13 D12S391 19,22 D2S441 13,15 -
STR鑒定圖
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參考文獻(xiàn)
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Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis(2024/01/17)
作者：Can Cheng, Hanhui Yao, Heng Li
期刊：Acta Pharmaceutica Sinica B
DOI：10.1016/j.apsb.2024.01.006
影響因子 ：14.500
引用產(chǎn)品：CL-0074
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Acetyltransferase NAT10 regulates the Wnt/β-catenin signaling pathway to promote colorectal cancer progression via ac4C acetylation of KIF23 mRNA(2022/12/15)
作者：Chi Jin, Tuo Wang, Dongsheng Zhang
期刊：JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
DOI：10.1186/s13046-022-02551-7
影響因子 ：11.300
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Ubiquitination-mediated degradation of SIRT1 by SMURF2 suppresses CRC cell proliferation and tumorigenesis(2020/05/02)
作者：Le Yu, Ling Dong, Hui Li
期刊：Oncogene
影響因子 ：8.000
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Mollugin suppresses proliferation and drives ferroptosis of colorectal cancer cells through inhibition of insulin-like growth factor 2 mRNA binding protein 3/glutathione peroxidase 4 axis(2023/09/05)
作者：Wei Wang, Lijiang Zhou, Xinyu Zhang
期刊：BIOMEDICINE & PHARMACOTHERAPY
DOI：10.1016/j.biopha.2023.115427
影響因子 ：7.500
引用產(chǎn)品：CL-0074
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Norcantharidin Sensitizes Colorectal Cancer Cells to Radiotherapy via Reactive Oxygen Species–DRP1-Mediated Mitochondrial Damage(2024/03/14)
作者：Qiong Xu, Heng Zhang, Haoren Qin
期刊：Antioxidants
影響因子 ：7.000
引用產(chǎn)品：CL-0074
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METTL3-mediated RanGAP1 promotes colorectal cancer progression through the MAPK pathway by recruiting YTHDF1(2024/01/24)
作者：Rui Yang, Cheng Yang, Danjie Su
期刊：CANCER GENE THERAPY
DOI：10.1038/s41417-024-00731-5
影響因子 ：6.400
引用產(chǎn)品：CL-0074
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Identification and validation of a novel prognosis model based on m5C-related long non-coding RNAs in colorectal cancer(2023/09/05)
作者：Ziyang Di, Gaoran Xu, Zheyu Ding
期刊：Cancer Cell International
DOI：10.1186/s12935-023-03025-2
影響因子 ：5.800
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Autophagy Induction by Trichodermic Acid Attenuates Endoplasmic Reticulum Stress-Mediated Apoptosis in Colon Cancer Cells(2021/5/25)
作者：Junyan Qu, Cheng Zeng, Tingting Zou
期刊：International Journal Of Molecular Sciences
影響因子 ：5.600
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SERPINB5 promotes colorectal cancer invasion and migration by promoting EMT and angiogenesis via the TNF-α/NF-κB pathway(2024/03/08)
作者：Bixia Liu, Yang Xie, Jiayu Zhang
期刊：INTERNATIONAL IMMUNOPHARMACOLOGY
DOI：10.1016/j.intimp.2024.111759
影響因子 ：5.600
引用產(chǎn)品：CL-0074
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Raltitrexed as a synergistic hyperthermia chemotherapy drug screened in patient-derived colorectal cancer organoids(2021/8/15)
作者：Lisi Zeng, Quanxing Liao, Haoran Zhao
期刊：Cancer Biology & Medicine
DOI：10.20892/j.issn.2095-3941.2020.0566
影響因子 ：5.500
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miR-145-5p restrained cell growth, invasion, migration and tumorigenesis via modulating RHBDD1 in colorectal cancer via the EGFR-associated signaling pathway(2019/11/03)
作者：Ying Niu, Jinping Zhang, Yalin Tong
期刊：International Journal Of Biochemistry & Cell Biology
DOI：10.1016/j.biocel.2019.105641
影響因子 ：4.000
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Integrated Multi-omics Analyses Identify CDCA5 as a Novel Biomarker Associated with Alternative Splicing, Tumor Microenvironment, and Cell Proliferation in Colon Cancer Via Pan-cancer Analysis(2024/01/01)
作者：Xinyue Bao, Xin Leng, Tianyu Yu
期刊：Journal of Cancer
影響因子 ：3.900
引用產(chǎn)品：CL-0074
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Critical role of guanylate binding protein 5 in tumor immune microenvironment and predictive value of immunotherapy response.(2022/09/30)
作者：Xiang Li, Dan Song, Song Su
期刊：Frontiers in Genetics
影響因子 ：3.700
引用產(chǎn)品：CL-0074
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HSPA5 repressed ferroptosis to promote colorectal cancer development by maintaining GPX4 stability(2022/09/01)
作者：Ronglin Wang, Lei Hua, Peixiang Ma
期刊：NEOPLASMA
DOI：10.4149/neo_2022_220331N363
影響因子 ：3.409
引用產(chǎn)品：CL-0074
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CAMK2N1 has a cancer-suppressive function in colorectal carcinoma via effects on the Wnt/β-catenin pathway(2022/08/15)
作者：Xiaolong Zhang, Lifei Tian, Zeyu Li
期刊：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
DOI：10.1016/j.bbrc.2022.08.036
影響因子 ：3.100
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RNF114 facilitates the proliferation, stemness, and metastasis of colorectal cancer(2023/07/27)
作者：Shicheng Liu, Lelan Gong, Fengchang Huang
期刊：PATHOLOGY RESEARCH AND PRACTICE
影響因子 ：2.800
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Circ_KIAA1199 inhibits MSI1 degradation by targeting miR-34c-5p to drive the malignant cell behaviors and tumor growth of colorectal cancer(2022/01/01)
作者：Yanbo Zhang, Hailong Yu, Zhen Guo
期刊：Anti-Cancer Drugs
DOI：10.1097/CAD.0000000000001164
影響因子 ：2.300
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產(chǎn)品資料
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![NCI-H446 [H446] (人小細(xì)胞肺癌細(xì)胞) (STR鑒定正確)](/Public/upfile/pic/xbx/CL-0401-1.jpg)
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